Objective To construct zebrafish uba6 and uba7 mutants. Method In-dels were introduced into coding regions of zebrafish uba6 and uba7 gene by TALEN technology, and mutation types were identified by sequencing. Results We obtained two uba6 and three uba7 mutants with different small fragment deletions resulting in reading frame shifts. Conclusion These uba6 and uba7 mutants provided starting materials for the study of zebrafish uba6 and uba7 gene functions.