Embryonic Stem cell Test(EST) is an alternative testing method for testing embryotoxicity and developmental toxicity which have been validated by the European Centre for Validation of Alternative Methods(ECVAM).Under the promote of new technology, EST method has been refined by many ways including gene transfection, utilizing human-derived stem cell, increase the assay throughput, integrating test endpoints and quantifying the test indexs,which enhanced scientific relevance and testing efficiency.Within the principle of integration testing strategy(ITS),conducting pre-validation tests and standardized experiments on a large scale of chemicals, the new EST method as a individual test or a part of integrated test systerm is promising to apply on the research of lead drugs and the embryotoxicity prediction and mechanism study of chemicals.