用压力-容积环评价大鼠心功能方法的改进及注意事项
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广州市重大专项科技项目(201300000051)。


Improvements and key points in the assessment of rat cardiac function using pressure-volume loop
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  • LIU Xiao-yu

    LIU Xiao-yu

    School of Bioscience and Bioengineering, South China University of Technology, Pre-Incubator for Innovative Drugs and Medicine Center, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China
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  • TAN Wen

    TAN Wen

    School of Bioscience and Bioengineering, South China University of Technology, Pre-Incubator for Innovative Drugs and Medicine Center, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China
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    摘要:

    目的 探讨应用压力-容积环(pressure-volume loop,PV Loop)评价大鼠心功能过程中的影响因素及方法改进原则,分析主动脉弓缩窄(transverse aortic constriction,TAC)诱导的压力负荷对大鼠心脏功能的影响,从而为大鼠健康或疾病状态下的心功能分析提供更为科学全面的方法。 方法 采用压力容积传感器从大鼠的颈动脉插入左心室,调整传感器到合适位置来改善压力-容积环的形状,结合配套软件对心功能进行多方面的评价,分别从压力及容积的定标、导管在左心室腔内的位置、呼吸机对测定的影响等方面分析应用压力-容积环测定大鼠心功能时的操作要点。结果 (1)通过颈静脉注射适量的高渗盐水定标,扣除心室壁平行电导率引起的容积,可以获得准确的左心室容积。(2)适度调节压力-容积导管,使传感器的位置完全进入心室但又不接触心室壁,可以得到更好的压力-容积信号。(3)停止呼吸机适当时间,消除非自主呼吸对测定的影响,有助于获得更稳定的数据。采用这一方法对主动脉缩窄手术(诱导心肌肥大)后1个月的大鼠进行分析,可以更好地评价大鼠在正常和疾病状态下的心功能。 结论 本研究从容积定标、导管位置和呼吸机等方面对大鼠心脏的压力-容积环测定方法进行了改进和完善,并通过分析正常和心肌肥大的大鼠心功能验证了该方法的可靠性。

    Abstract:

    Objective To investigate the key factors for PV Loop evaluation in rats and to improve this method. To provide examples of cardiac function measurements obtained from normal rats and from rats with cardiac hypertrophy induced by transverse aortic constriction (TAC). To establish a more reliable method for rats heart measurements. Methods Rats underwent left ventricular catheterization through the right carotid artery. Through adjustments of the position of the pressure-volume conductance catheter, the optimal PV Loops and a number of cardiac functional parameters were acquired. The key influencing factors, calibration of volume, position of the catheter in the left ventricle (LV) and suspension of ventilator were assessed. Results 1. The real volume of left ventricle were acquired by injecting appropriate volume of hypertonic saline through jugular vein, which deducted the parallel conductivities of ventricular wall. 2. To get better PV loops, it's important to adjust the position of the catheter in the left ventricle until all of the pressure and volume sensors were located in the ventricle as well as out of touch with the ventricular wall. 3. Suspension the ventilator during the test is conducive to stable and reasonable data acquisition. We further assessed the cardiac functions of healthy rats and rats with cardiac hypertrophy with this improved method, which showed better performances. Conclusions This study we have evaluated the influences of calibration of volume, position of the catheter in the left ventricle (LV) and ventilator on measurements of rats PV loops, and further improved this method. Moreover, we have validated this method with measurements of cardiac functions of normal rats and cardiac hypertrophic rats.

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刘晓雨,谭文.用压力-容积环评价大鼠心功能方法的改进及注意事项[J].中国比较医学杂志,2016,26(6):75~82,87.

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  • 最后修改日期:2016-03-09
  • 在线发布日期: 2016-06-30
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