Objective To prove the advantages of telemetry by comparing with the traditional methods in safety pharmacology. Methods To monitor continuously the heart rate, respiratory rate, blood pressure and ECG of Beagle dogs by traditional and telemetry methods respectively, analyze and compare the changes between anesthetized and conscious dogs before and after feeding. Results Maintenance of anesthesia changed significantly the heart rate, respiratory rate, blood pressure and QT interval in the ECG of animals. The changes of physiological indicators in 24 h is not obvious in conscious animals, and showed a certain biorhythm. Compared with the conscious animals, the anesthetized dogs' heart rate was significantly higher, blood pressure increased significantly, QRS and QTcf interval prolonged significantly, respiratory frequency decreased, heart rate increased significantly after feeding, and QTcf interval extended very significantly. Conclusions Traditional methods in safety pharmacology affect animal physiological indicators such as heart rate, blood pressure, respiratory rate and QT interval, which affect the objectivity of drug evaluation. Using conscious animals by telemetry can reduce these errors, however, the interference from outside should be eliminated.