Objective Study on the blood pressure, renal sodium excretion, related renal sodium ion channels and signaling pathways between male and female mice, to investigate the relationship between gender differences in blood pressure and renal sodium ion channel.Methods Blood pressure of adult female and male mice was measured using tail-cuff sphygmomanometer. Plasma and urine electrolytes were performed using flame photometer.Protein levels of renal sodium transporters NCC, NKCC2, ENaC were determined by western blot, mRNA levels of these transporters and the upstream With No lysine (WNK) kinases were determined by Real-time PCR.Results The blood pressure of female mice was significantly lower than that of male mice. Renal sodium excretion and mRNA levels of NCC&NKCC2 were increased significantly in female mice. No obvious difference was observed in ENaC. The mRNA level of WNK4 was increased while no change in WNK1.Conclusions The lower blood pressure of female mice may caused by increasing renal sodium excretion and WNK4-NCC/NKCC2 signaling pathway involved.