Objective To investigate biological parameters and morphology in spontaneous type 2 diabetes C57BL/KsJ-db/ db mice and mice with type 2 diabetes mellitus which induced by high-fat diet and streptozotocin injections. Methods C57BL/6J mice were divided randomly into normal control group, Induced Group, and Spontaneous Group with 10 mice in each group; Induced Group: The 11 weeks old C57BL/6J mice were fed on a high-fat diet before intraperitoneal injections of STZ; Spontaneous Group: 8 weeks old C57BL/KsJ-db/db mice (db/db); The body weight, Fasting blood glucose (FBG), oral glucose tolerance test (OGTT) was detected every week for 28 days. FINS, AUC, IR index and pro-inflammation factors (TNF-α, IL-18, IL-1β, INF-γ) were evaluated in the beginning and 8^th week. At the end of experiment, the mice were sacrificed for organ and morphological study. Results The weight of Spontaneous Group were higher than Induced Group, both of them are higher than normal group significantly; Non-fasting blood glucose concentrations of Spontaneous Group mice were higher over Induced Group; In 8^th week the abnormal glucose levels of Spontaneous Group extraordinary stability, however, in Induced Group drops obviously;the FINS was increased in Spontaneous Group, but have no significantly change in Induced Group,both AUC and HOMA-IR index Show a significant downtrend in Induced Group, However, in Spontaneous Group, the rising trend of AUC and HOMA-IR are stabe; The content of the pro-inflammatory factor (TNF-α, IL-18, IL-1β, INF-γ) in both diabetes animal are more than normal group. In 8th week the content of pro-inflammatory factor was decreased in Induced Group, but increased in Spontaneous Group significantly; Pathologic results showed that pancreas, kidney, liver, muscle, and testis, appeared serious inflammatory pathological deterioration in two kinds of diabetes models mice. Conclusions During 8weeks the Spontaneous diabetes animal showed abnormal glucose metabolism, and high concentration of pro-inflammatory cytokines which Symptoms of type 2 diabetes become more and more serious.; glucose metabolism and inflammation showed improved in induced animal model.