Objective To explore effect of Xylazine hydrochloride on Bama minipigs under general anesthesia. To emphasize safety consciousness of general anesthesia. To research cardiac main function and structure of normal Bama minipigs in preparation for the subsequent comparative medicine research. Methods 43 Bama minipigs,inject in post aurem muscles of neck with 5 mL of mixed drug conclude Xylazine hydrochloride (2 mL), Atropine Sulfate(1 mL) and Droperidol(2 mL) on each one. Echocardiography after general anesthesia.Observe induction and recovery time of anesthesia, anesthesia maintaining time, total check time and the others. Introduce the method of simple endotracheal intubation.Results Anesthesia, induction period (18±3)min, maintaining period (40±5)min, recovery period (60±10)min. Echocardiography, LAD (2.54±0.20) cm, LVDd (3.41±0.25) cm, LVDs (2.28±0.23) cm, IVSTd (0.60±0.07) cm, LVPWTd (0.59±0.07) cm, AoD (1.77±0.18) cm, EDV (48.59±8.31) cm, ESV (18.28±4.46) mL, SV (39.30±5.16) mL, LVEF (62.76±5.01)%. Conclusions Intramuscular injection of xylazine hydrochloride with droperidol and atropine sulfate on bama minipigs for general anesthesia is a highly conserved specie in cardiovascular system and safe. We obtained some information of cardiac main function and structure of normal Bama minipigs which could provide reference for scientific research and veterinarian clinic.