Objective To improve the method of paraffin section and frozen section of adipose tissues. Methods The adipose tissues were collected and quickly placed in 10% neutral formaldehyde. Sections were prepared by setting different dehydration procedures. The slices were observed under microscope after Hematoxylin-Eosin staining. The expression of PPARγ and ITLN1 in adipose tissues was detected by immunohistochemistry. For frozen sectioning, the adipose tissues were collected and quickly placed in 10% neutral formaldehyde or Calcium formaldehyde, After embedded by OCT, put in -80℃ refrigerator for at least 30 minutes. Setting the temperature of frozen section cabinet to -20℃ and the temperature of sample to -30℃. Results Following the improved methods, the slices of adipose tissues were better than before. The structural integrity and consistency of adipose tissues were preserved well, the staining was distinct, and the improved methods had no effect on immunohistochemistry results. The improvement of the frozen sectioning had greatly improved the slice quality, the adipose tissues showed complete structure and good morphology. Conclusions The improved methods can be successfully applied to different experimental animals, which provide a powerful condition for the study of adipose tissues.