国家自然科学基金项目（81500083,81371945,81572159,81101342）；空军总医院院级课题（KZ2014023）；北京市自然科学基金项目（7132133）。
目的 研究小鼠脾脏间充质干细胞（Sp-MSCs）对T淋巴细胞增殖与活化的调节作用。方法 采用组织块法从小鼠脾脏中分离培养出Sp-MSCs，并做多向诱导分化实验。分离小鼠T淋巴细胞，开展淋巴细胞转化试验和混合淋巴细胞反应，分别检测Sp-MSCs对于非特异抗原和同种异体抗原活化的T淋巴细胞的影响。以CFSE流式法检测Sp-MSCs对活化的T淋巴细胞增殖的影响。采用定量PCR分析Sp-MSCs对T淋巴细胞表达的炎性细胞因子的影响。结果 流式检测结果显示Sp-MSsC可以抑制非特异抗原和同种异体抗原引起的T淋巴细胞增殖。Sp-MSCs能够有效抑制淋巴细胞转化试验和混合淋巴细胞反应引起的细胞活化。进一步分析发现，Sp-MSCs抑制T淋巴细胞表达干扰素γ，肿瘤坏死因子α和白细胞介素17A。结论 Sp-MSCs能够抑制T淋巴细胞增殖与活化，抑制T淋巴细胞表达多种炎性细胞因子。
Objective To investigate the modulatory effects of mouse spleen-derived mesenchymal stem cells (Sp-MSCs) on the proliferation and activation of T lymphocytes.Methods The mouse Sp-MSCs were isolated from mouse spleens by using explant tissue culture protocol and the cells were induced to differentiate into osteoblasts and adipocytes. The effects of Sp-MSCs on proliferation of activated T lymphocytes were determined by carboxyfluorescein succinimidyl ester (CFSE) staining assay. Furthermore, the effects of Sp-MSCs on lymphocyte transformation test (LTT) and mixed lymphocytes reaction (MLR) were observed, respectively. In addition, the mRNA expression of T cell derived proinflammatory cytokines by Sp-MSCs was detected by quantitative PCR.Results The results of CFSE assay demonstrated that Sp-MSCs suppressed ConA and allogeneic lymphocyte-induced T cell proliferation. Moreover, the Sp-MSCs were capable of suppressing LLT and MLR. Additionally, the results of quantitative PCR revealed that Sp-MSCs significantly suppressed the T cells expressing interferon-γ, tumor necrosis factor-α and interleukine-17A.Conclusions Sp-MSCs are capable of suppressing the proliferation and activation of T lymphocytes as well as the expression of T cell-derived proinflammatory cytokines.
丁丽,朱恒,张海宏,杨洋,韩冬梅,王志东,郑晓丽,董磊,闫洪敏,刘静,朱玲,薛梅,郭子宽,王恒湘.小鼠脾脏间充质干细胞调节T淋巴细胞的增殖与活化[J].中国比较医学杂志,2017,27(2):15~20.
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