Objective To establish a dog model of hyperdynamic septic shock by intravenous injection of Escherichia coli. Methods Twenty-two hybrid dogs were randomly divided into control group (n=11) and experimental group (n=11). A double-cavity central venous catheter was inserted into the right femoral vein of the dogs after anesthesia. Physiological saline was continuously pumped into the femoral vein of the dogs in the control group by the micro-pump, while the dogs of the septic shock group were pumped with E.coli in the same way. The catheter of PICCO (pulse indicator continuous cardiac output) was inserted into the right femoral artery and the systemic hemodynamics of the dogs was monitored at all of the time points from 0 to 48 h. Results Compared with the control group, the heart rate (HR), cardiac output (CO) and stroke volume (SV) of the experimental group were significantly increased at 12 h (P < 0.05), and the systolic blood pressure (SBP) and systemic vascular resistance (SVR) were significantly decreased at 12 h (P < 0.05). However, other hemodynamic parameters such as the central venous pressure (CVP), the pulmonary artery wedge pressure (PAWP), the oxygen delivery rate (DO₂), the oxygen consumption rate (VO₂), and the oxygen extraction rate (O₂ER) were not significantly different (P > 0.05). Moreover, significant decrease in urine output was observed in the septic shock group since 12 hours, and the peak systolic velocity (PSV) and the resistance index (RI) of the systolic artery were significantly different from those in the control group (P < 0.05). Conclusions The dog model of hyperdynammic septic shock can be successfully established by intravenous injection of Escherichia coli into the femoral vein by a micro-pump. This model provides an experimental basis for further study of septic shock.