(1. 贵阳中医学院第一附属医院检验科,贵阳 550001; 2. 贵州医科大学医学检验学院,贵阳 550004)
R-33
贵州省科技厅项目(黔科合LH 字[2016] 7511 号);贵州省教育厅青年科技人才成长项目(黔教合KY [2016] 190)
(1. Department of Clinical Laboratory, the First Affiliated Hospital of Guiyang University of Chinese Medicine,Guiyang 550001, China; 2. School of Clinical Laboratory Science, Guizhou Medical University, Guiyang 550004)
目的构建雄性和雌性氟中毒大鼠动物模型,探讨氟中毒性别差异性?方法 选择48 只体重150 g左右清洁级SD 大鼠,分为对照组和染氟组?模型组自由食用含氟饲料,饲料含氟量100 mg/ kg?每三天动态观察大鼠日常情况?体重?氟斑牙的变化,分别于70 d 雌性大鼠和110 d 雄性大鼠全部出现氟斑牙时股动脉取血处死一批大鼠,采用氟离子选择电极法检测大鼠尿氟和骨氟含量?结果 对照组雌雄大鼠实验全过程均未出现氟斑牙?染氟组雌鼠和雄鼠首次出现肉眼氟斑牙时间在染氟60 d 和80 d 左右,雄鼠首次出现氟斑牙比雌鼠晚约20 d?染氟30 d 左右雌雄大鼠进入快速生长期,雌雄大鼠体重差异有显著性(P < 0.01)?随染氟时间延长,110 d 染氟组雄鼠和雌鼠体重高于对照组?随染氟时间延长,雌雄大鼠尿氟和骨氟逐渐升高,染氟组明显高于对照组,差异有显著性(P <0.01),尿氟和骨氟与染氟时间呈正相关?雄鼠尿氟和骨氟高于雌性,差异有显著性(P < 0?? 01),尿氟和骨氟受性别的影响?结论 构建氟中毒雌雄大鼠动物模型的体重?出现氟斑牙时间?尿氟和骨氟水平存在性别差异?
Objective To construct the male and female rat model of fluorosis and to explore the gender difference in fluorosis. Methods A total of 48 clean?grade SD rats with the body weight of about 150 g (male∶ female = 1∶ 1) were equally divided into the control group and fluorosis model group. Rats in the fluorosis model group were fed with fluorine-containing (100 mg/ kg) feedstuff. The general condition, changes in body weight and dental fluorosis of the rats were observed every three days. A batch of female and male rats were sacrificed by femoral artery bleeding on the 70th and 110th day of experiment, respectively, when all rats showed dental fluorosis. The levels of urinary fluoride and bone fluoride of the rats were measured by a fluoride ion-selective electrode. Results All the male and female rats in the control group did not show dental fluorosis during the entire experiment. The female and male rats in the fluorosis group showed dental fluorosis visible to naked eye began approximately on the 60th and 80th day, respectively,indicating that the appearance of dental fluorosis of the male rats was about 20 days later than the female rats. After 30 days or so, the male and female rats went into a rapid growth period, and there were significant differences between the body weights of the male and female rats (P < 0.01). The body weight of the male and female rats in the fluorosis group was higher than that of the control group on the 110th day. With the progression of fluorosis, the levels of urinary fluoride and bone fluoride of the rats were gradually increased, and finally significantly higher than those of the control group (P < 0.01). There was a positive correlation between the levels of urinary and bone fluoride and the time of fluorosis. The levels of urinary and bone fluoride of the male rats were significantly higher than those of the female rats (P < 0.01), indicating that they are affected by gender. Conclusions The body weight of the rat model of fluorosis, the starting time when dental fluorosis occurs and the levels of urinary and bone fluorine are all different between male and female rats.
刘洋,韩伦英,何川,彭凤涛,李桂筑,王飞清.不同性别氟中毒大鼠模型的比较研究[J].中国比较医学杂志,2018,28(1):33~37.
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