Objective To measure and analyze the behavioral changes of Rncat congenital cataract mice. Methods Normal BALB/ c mice and KM mice were used as control group, and inbred and random mated Rncat congenital cataract mice were used as experimental group. Behavioral tests, including the open field test, coat-hanger test, forced swimming test, and tail suspension test, were conducted on the mice. Results Compared with the inbred Rncat congenital cataract mice, the residence time in the open field test, the immobility time in the forced swimming test and tail suspension test of the BALB/ c mice, randomly-mated Rncat congenital cataract mice and KM mice were significantly different. Conclusions There are certain differences in behavioral performance between the Rncat congenital cataract mice and the other mice. Our findings may provide a useful reference for future researchers.