杏仁核快速电点燃癫痫大鼠神经元限制性沉默因子表达变化
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(徐州医科大学人体解剖学教研室,江苏徐州 221004)

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R-33

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江苏省教育厅科研项目(编号:14KJB310021)


Changes in expression of repressor element silencing transcription factor in rapid amygdala kindling epilepsy rats
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(Department of Human Anatomy, Xuzhou Medical University, Xuzhou 221004, China)

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    摘要:

    目的 研究杏仁核快速电点燃癫痫中神经元限制性沉默因子(repressor element silencing transcription factor,REST)表达变化?方法 成年Sprague-Dawley(SD)大鼠随机分为假手术组(S 组)?癫痫模型2 h 组(EP-2 h 组)?癫痫模型14 d 组(EP-14 d 组)和癫痫模型35 d 组(EP-35 d 组);癫痫采用杏仁核快速电点燃刺激法建立大鼠癫痫模型,癫痫模型成功后2 h?14 d 及35 d 时,灌注?固定?冰冻切片,行神经元限制性沉默因子(REST)及神经元核蛋白(neuron specific nuclear protein,NeuN)免疫组化学染色?结果 与S 组比较,EP 各组大鼠海马区REST 表达均上调,且EP-2 h 组上调最明显( P < 0.05);而EP 各组大鼠海马区NeuN 的表达降低,有明显的神经元丢失,35 d 最明显?结论 杏仁核快速电点燃癫痫的形成可能与REST 表达上调有关?

    Abstract:

    Objective To detect changes in expression of repressor element silencing transcription factor (REST) in rapid amygdala kindling epilepsy rats. Methods Adult Sprague-Dawley rats were randomly divided into a sham group (group S), epilepsy 2 h group (group EP-2 h), epilepsy 14 d group (group EP-14 d), and epilepsy 35 d group (group EP-35 d). An experimental epilepsy kindling model of the amygdala was established by electrical stimulation. After 2 h, 14 d, and 35 d, rats were perfused and their brains were fixed and frozen for coronal sections. Immunohistochemistry was used to detect expression of REST and the neuron specific nuclear protein, NeuN. Results Expression of REST was significantly increased in the CA1, CA3, and dentate gyrus regions in groups EP compared with group S ( P < 0.05), particularly in group EP-2 h. In contrast, NeuN staining was significantly decreased in groups EP compared with group S. Visible neuronal loss was observed in the hippocampus of the EP-35 d group. Conclusions Up-regulation of REST in rat hippocampus may be involved in epileptogenesis.

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呼奶英,于佳田,别会杰,王德广.杏仁核快速电点燃癫痫大鼠神经元限制性沉默因子表达变化[J].中国比较医学杂志,2018,28(5):6~11.

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  • 收稿日期:2017-10-30
  • 在线发布日期: 2018-06-26