(首都医科大学宣武医院中心实验室,北京市老年病医疗研究中心,北京脑重大疾病研究院阿尔茨海默病研究所,神经变性病教育部重点实验室,北京 100053)
R-33
国家重点研发计划资助项目(编号:2016YFC1306300);北京市医院管理局临床医学发展专项经费(扬帆计划) (编号:ZYLX201706)
KANG Meimei
Department of Central Laboratory, Xuanwu Hospital, Capital Medical University; Beijing Geriatric Medical Research Center; Center of Alzheimer’s disease, Beijing Institute for Brain Disorders; Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, ChinaWANG Rong
Department of Central Laboratory, Xuanwu Hospital, Capital Medical University; Beijing Geriatric Medical Research Center; Center of Alzheimer’s disease, Beijing Institute for Brain Disorders; Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China(Department of Central Laboratory, Xuanwu Hospital, Capital Medical University; Beijing Geriatric Medical Research Center; Center of Alzheimer’s disease, Beijing Institute for Brain Disorders; Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China)
2D 细胞培养的细胞在体外环境下随着增生会逐渐丧失原来的性状;动物模型实验繁琐,价格昂贵;而3D 细胞培养模型可在一定程度上弥补动物模型和2D 细胞模型的缺陷,越来越受到大家重视?2014 年数据报道阿尔茨海默病(Alzheimer’s disease,AD)65 岁以上人群发病率在5.14%,我国AD 患者2016 年已达800 万,但该病的发病机制尚未明确,对AD 的研究一直是热点及难点?本文将简单介绍2D 细胞培养和3D 细胞培养,并对3D细胞培养近些年在AD 研究方面的应用作一综述?
Cells in two-dimensional (2D) cultures gradually lose their original traits as they are passaged in vitro. Existing animal models are expensive and animal experiments require too much work to conduct large-scale experiments. Three-dimensional (3D) cell culture models have attracted increasing attention as they can circumvent the limitations of these previous two models. In 2014, it was reported that the incidence of Alzheimer’s disease (AD) in people over 65 years old was 5.14%. In China, the number of AD patients reached 8 million in 2016, but the pathogenesis of the disease is not yet clear. Study of AD is a ‘hot’ yet complicated issue. This article will briefly introduce 2D and 3D cell cultures and the application of 3D cell culture to AD research in recent years.
康美美,王蓉.3D 细胞培养在阿尔茨海默病研究中的应用[J].中国比较医学杂志,2018,28(5):12~15.
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