(广东药科大学,基础学院血管生物学研究所,广州 510006)
R-33
国家自然科学基金(31500966,81773095);广州市科技计划项目(201607010135);广东省科技计划项目(2015A020211029, 2014A020212314)
(Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, China)
目的 探讨心房钠尿肽(atrial natriuretic peptide, ANP) 对黑色素瘤肺转移的作用?方法 将体外培养的黑色素瘤B16F10 细胞株通过尾静脉注射到心房钠尿肽敲除小鼠(ANP - / - 小鼠)和C57BL/6J 小鼠的体内,建立黑色素瘤肺转移模型,模型建立后第21 天计数转移到肺表面的结节数目,并把肺组织包埋固定?切片?HE 染色后,确定肺转移结节并计数?结果 ANP 敲除小鼠肺表面转移的黑色素瘤结节数明显比C57BL/6J 小鼠少,差异有显著性;ANP 敲除小鼠肺微转移结节数目也明显比C57BL/6J 小鼠少,差异有显著性?结论 ANP 敲除显著抑制了黑色素瘤的肺转移?
Objective To investigate the role of atrial natriuretic peptide (ANP) in lung metastasis of melanoma. Methods Melanoma B16F10 cells were intravenously injected into ANP knockout mice and C57BL/6J mice. After threeweeks, the mice were sacrificed, and the lungs were removed, embedded, and examined by pathology using HE staining. The numbers of metastatic foci on the lung surface and micrometastatic foci in the lung tissues were counted. Results The ANP knockout mice displayed fewer metastatic foci on the lung surface and less micrometastatic foci in the lung tissues of ANP knockout mice than in the C57BL/6J mice. Conclusions ANP deletion significantly suppresses the metastasis of melanoma in the lung of mice.
亓翠玲,曹静桦,何亚军,李倩明,李梦诗,杨扬,王丽京.心房钠尿肽在基因敲除小鼠黑色素瘤肺转移中的作用[J].中国比较医学杂志,2018,28(6):1~3.
复制