消癌平注射液对原发性肝癌模型大鼠病理形态和肝癌细胞迁移的影响及其作用机制
作者:
作者单位:

(首都医科大学附属北京世纪坛医院,北京 100038)

中图分类号:

R-33

基金项目:

北京市中医管理局中西医结合项目(2014?ZYJ09)


Effect of a Chinese medicine Xiao Aiping injection on the primary liver cancer pathology and the cancer cell migration ability in rat models, and their mechanism of action
Author:
Affiliation:

(Beijing Shijitan Hospital, Capital Medical University, Beijing 100038,China)

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    摘要:

    目的 探讨消癌平注射液对间断性腹腔注射二乙基亚硝胺复制肝癌模型大鼠肝组织病理损伤的影响及其在低氧肿瘤微环境中对肝癌细胞迁移的作用及机制?方法 选取SD 大鼠60 只,随机分为模型组48 只?正常对照组12 只?模型组给予间断性腹腔注射二乙基亚硝胺建立肝癌模型,正常对照组腹腔注射等量生理盐水?14 周后,将模型组随机均分为三组,两组分别腹腔注射消癌平注射液低剂量及高剂量,一组及正常对照组腹腔注射等量生理盐水,每周5 d,连续4 周?第19 周采集标本,检测大鼠肝组织病理学改变?在CoCl 2 诱导的低氧环境下通过划痕愈合实验观察消癌平注射液对肝癌细胞SMMC?7721 和HepG2 迁移的影响,同时检测消癌平对肝癌细胞IL?6 mRNA 表达的影响?结果 与正常对照组相比,模型组大鼠肝组织呈明显肝癌样变,说明肝癌模型复制成功?治疗后,与模型组比较,消癌平低剂量和高剂量组大鼠肝组织病理损伤减轻;消癌平注射液在低氧环境下能够显著抑制HepG2 细胞的迁移,抑制其IL?6 mRNA 的表达,同等浓度下只能抑制SMMC?7721 细胞的迁移,具有抑制其IL?6 mRNA 表达的作用趋势但差异无统计学意义?结论 消癌平注射液能够减轻间断性腹腔注射二乙基亚硝胺诱导的原发性肝癌模型大鼠肝组织的病理损伤,在化学诱导的低氧环境下对SMMC?7721 和HepG2 细胞迁移的抑制作用不同,下调IL?6 mRNA 的表达可能是其发挥抑制肝癌细胞迁移的作用机制之一?

    Abstract:

    Objective To investigate the effects of a Chinese medicine Xiao Aiping injection on the pathological changes of liver tissues in rat models, the migration ability of cancer cells in hypoxic tumor microenvironment, and explore their mechanism of action. Methods Sixty SPF 5?week old male Sprague?Dawley rats were randomly divided into model group (n=48) and blank control group (n=12). The model group was intraperitoneally injected with diethylnitrosamine to establish hepatocellular carcinoma model, and the blank control group was injected equal amount of normal saline. After 14 weeks, the model group was equally divided into three groups, two of them received the Xiao Aiping injection at a low and high dose, respectively, and the rest of model group and the blank control group were injected with normal saline once per day for five days, lasting for consecutive four weeks. Liver specimens were collected at the nineteenth week, and the rat liver cancer and liver tissues were examined by pathology. In the hypoxic environment induced by CoCl2 , the effect of Xiao Aiping injection on the migration ability of hepatocellular cancer SMMC?7721 and HepG2 cells was assessed by wound healing experiment, and the expression of IL?6 mRNA was detected by real?time PCR assay. Results Compared with the blank control group, hepatic tissue of the model group showed obvious hepatocellular carcinoma?like?changes indicating the successful establishment of hepatocellular carcinoma model. After treatment, compared with the model group, the pathological alterations of hepatic tissue of Xiao Aiping injection low and high doses groups were reduced, and were more obvious in the high dose group. Under hypoxic condition, Xiao Aiping injection obviously inhibited the migration of HepG2 cells and inhibited IL?6 expression in the liver tissues. With the same concentration, Xiao Aiping injection obviously inhibited the migration of SMMC?7721 cells, and showed an inhibiting trend of IL?6 mRNA expression, but the difference was not statistically significant. Conclusions Xiao Aiping injection can reduce the pathological changes of hepatocellular carcinoma in rat models induced by intermittent intraperitoneal injection of diethylnitresamine. In the chemically induced hypoxic environment, the effects of Xiao Aiping injection on the migration of SMMC?7721 and hepG2 cells are different. Downregulating the expression of IL?6 mRNA may be one of the mechanisms of migration inhibiting effects.

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温丽娜,郭杨志,仝永娟,李莎.消癌平注射液对原发性肝癌模型大鼠病理形态和肝癌细胞迁移的影响及其作用机制[J].中国比较医学杂志,2018,28(6):46~52.

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  • 收稿日期:2017-12-28
  • 在线发布日期: 2018-07-20
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