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首页 > 过刊浏览>2018年第28卷第6期 >65-71. DOI:10.3969/j. issn. 1671 -7856. 2018. 06. 012
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Galectin 家族成员对HIV⁃1 感染巨噬细胞凋亡的影响
作者:
  • 王永芳

    王永芳

    北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
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  • 彭卓颖

    彭卓颖

    北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
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  • 李想

    李想

    北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
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  • 丛喆

    丛喆

    北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
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  • 薛婧

    薛婧

    北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
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  • 魏强

    魏强

    北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
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作者单位:

(北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021)

中图分类号:

R-33

基金项目:

中国医学科学院医学与健康科技创新工程重大协同创新项目(2017?I2M?1?014); 十三五科技部传染病重大专项 (2017ZX10304402)


Effects of galectins on the apoptosis in HIV⁃1⁃infected macrophages
Author:
  • WANG Yongfang

    WANG Yongfang

    Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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  • PENG Zhuoying

    PENG Zhuoying

    Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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  • LI Xiang

    LI Xiang

    Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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  • CONG Zhe

    CONG Zhe

    Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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  • XUE Jing

    XUE Jing

    Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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  • WEI Qiang

    WEI Qiang

    Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
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Affiliation:

(Comparative Medicine Center, Peking Union Medical College (PUMC) & Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Sciences (CAMS); Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China)

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    摘要:

    目的 通过研究galectin?2?galectin?4?galectin?7?galectin?8?galectin?9 对HIV?1 感染巨噬细胞凋亡的影响,为清除HIV?1 感染巨噬细胞类型的病毒储存库提供参考依据?方法 用不同浓度galectin 家族成员诱导THP?1 细胞凋亡,探索galectins 的适宜浓度,利用PMA 将单核细胞(THP?1)刺激分化成为巨噬细胞(THP?1?Mφ),然后用制备的HIV?1 病毒感染巨噬细胞,最后选用适宜浓度的galectin?2?galectin?4?galectin?7?galectin?8 和galectin?9 分别处理未感染与HIV?1 感染的巨噬细胞并检测其凋亡情况?结果 针对巨噬细胞(THP?1?Mφ),未添加galectin 家族成员的对照组巨噬细胞(THP?1?Mφ)凋亡率为(4.39 ±0.74)%,5 μmol/ L galectin?2?5 μmol/ L galectin?4?7.5 μmol/ L galectin?7?3 μmol/ L galectin?8?1 μmol/ L galectin?9 分别诱导巨噬细胞(THP?1?Mφ)的凋亡率为(4.78±0.41)%?(7.21 ±1.46)%?(3.78 ± 1.03)%?(5.88 ± 2.08)%?(8.10 ± 4.13)%,各galectin 处理组与对照组相比,差异无显著性( P > 0.05)?针对HIV?1 感染的巨噬细胞(HIV?1?THP?1?Mφ),未添加galectin 家族成员的对照组HIV?1 感染巨噬细胞(HIV?1?THP?1?Mφ)凋亡率为(12.69 ± 1.16)%;5 μmol/ L galectin?2?5 μmol/ L galectin?4?7.5μmol/ L galectin?7?3 μmol/ L galectin?8?1 μmol/ L galectin?9 诱导HIV?1 感染的巨噬细胞(HIV?1?THP?1?Mφ)凋亡率分别为(11.69 ±0.90)%?(17.45 ±1.30)%?(32.01 ± 1.30)%?(15.77 ± 1.21)%?(19.27 ± 2.13)%,galectin?7 处理组与对照组相比,差异有显著性( P < 0.001)?结论 Galectin?7 不会引起未感染的巨噬细胞发生凋亡,却能特异性诱导HIV?1 感染的巨噬细胞发生大量凋亡?

    Abstract:

    Objective To investigate the effects of galectin?2, galectin?4, galectin?7, galectin?8, and galectin?9on the apoptosis in HIV?1?infected macrophages and to provide the theoretical and application basis for elimination of HIV?1?infected cellular reservoirs. Methods Firstly, apoptosis of human monocytic cell line THP?1 cells was induced bydifferent concentrations of galectins to determine the suitable concentration of different galetcins. Secondly, monocytes (THP?1) were stimulated to differentiate into macrophages (THP?1?Mφ) with phorbol myristate acetate (PMA), and then macrophages were prepared and infected with HIV?1. Finally, HIV?1?infected and uninfected macrophages were respectively treated with the suitable concentrations of galectin?2, galectin?4, galectin?7, galectin?8, galectin?9 and then the apoptosis in these macrophages was detected. Results The cell death rate of macrophages without treatment was 4.39 ±0.74%. The cell death rates of macrophages induced by 5 μmol/ L galectin?2, 5 μmol/ L galectin?4, 7.5 μmol/ L galectin?7, 3 μmol/ L galectin?8 and 1 μmol/ L galectin?9 were 4.78 ±0.41%, 7.21 ±1.46%, 3.78 ± 1.03%, 5.88 ±2.08%, 8.10 ±4.13%, respectively, with no statistically significant defferences among the groups ( P > 0.05). The cell death rate of HIV?1?infected macrophages without treatment was 12.69 ±1.16%, and that of HIV?1?infected macrophages induced by 5 μmol/ L galectin?2, 5 μmol/ L galectin?4, 7.5 μmol/ L galectin?7, 3 μmol/ L galectin?8 and 1 μmol/ L galectin?9 were 11.69 ±0.90%, 17.45 ±1.30%, 32.01 ±1.30%, 15.77 ±1.21% and 19.27 ±2.13%, respectively. There were significant differences between the control group and galectin?7?treated group ( P < 0.001). Conclusions Galectin?7?induces extensive apoptosis in HIV?1?infected macrophages but not in uninfected macrophages.

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王永芳,彭卓颖,李想,丛喆,薛婧,魏强. Galectin 家族成员对HIV⁃1 感染巨噬细胞凋亡的影响[J].中国比较医学杂志,2018,28(6):65~71.

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  • 收稿日期:2017-12-28
  • 在线发布日期: 2018-07-20
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