(1. 贵阳中医学院第一附属医院检验科,贵阳 550001; 2. 贵州医科大学附属乌当医院检验科,贵阳 550018; 3. 贵航300 医院检验科,贵阳 550002; 4. 贵州医科大学公共卫生学院,贵阳 550004)
R-33
贵州省科技厅项目(黔科合基础[2018]1015,黔科合LH 字[2017]7127 号?7128 号,黔科合LG 字[2011]036 号);贵州省卫生计生委科学技术基金项目(编号:gzwjkj2017?1?055);贵中医科院内[2017]14 号?
(1. Department of Clinical Laboratory, the First Affiliated Hospital of Guiyang University of Chinese Medicine, Guiyang 550001, China. 2. Department of Clinical Laboratory, Affiliated Wudang Hopital of Guizhou Medical University, Guiyang 550018.3. Department of Clinical Laboratory, Guihang 300 Hospital, Guiyang 550002 4. School of Public Health, Guizhou Medical University, Guiyang 550004)
目的 探讨钙?镁?铜?锌?维生素D 和蛋白联合干预燃煤型氟中毒对雌鼠卵巢功能变化?方法120只体重80 g 左右的清洁级SD 雌性大鼠为研究对象,随机分为对照组?染氟组?多种元素组?多种元素+ 蛋白组,每组30 只?各模型组大鼠自由食用不同配方的病区原煤加拌泥煤烘烤的玉米饲料?分别于染氟60,120,180 d 处死雌鼠,分离双侧卵巢?免疫组化法检测卵巢颗粒细胞凋亡蛋白Bcl?2 和Bax 的表达?HE 染色光镜下观察卵巢组织形态?各级卵泡数量变化?结果 卵巢颗粒细胞Bcl?2 蛋白表达量差异无显著性?各组与染氟组Bax 蛋白比较, 120 d 和180 d 差异有显著性( P < 0.05),180 d 两干预组比较差异有显著性( P < 0.05)?各组内比较,染氟组Bax蛋白表达量逐渐增多,差异有显著性( P < 0.05)?多种元素组60 d 与180 d 比较差异有显著性( P < 0.05)?染氟组随时间延长,卵巢颗粒细胞呈现渐进性水肿的变化,闭锁的卵泡和黄体退化逐渐增多?两干预组卵巢颗粒细胞少见轻度水肿,正常的各级卵泡多见,闭锁的卵泡和退化的黄体明显降低;与染氟组相比,60 d 对照组和多种元素+ 蛋白组闭锁卵泡降低,差异有显著性( P < 0.05);120 d 和180 d 对照组?两干预组差异有显著性( P < 0.05)?结论 钙?镁?铜?锌?维生素D 可拮抗燃煤型氟中毒对卵巢生殖毒性降低Bax 蛋白的表达量,降低闭锁卵泡数量,同时添加蛋白联合干预效果更佳?
Objective To study effects of Ca, Mg, Cu, Zn, VD combined with protein on ovarian functions in female rats with coal?burning fluorosis. Methods A total of 120 clean?grade SD female rats with body weights of about 80 g were used. Rats were randomly divided into control, fluorosis, element, and element + protein groups with 30 rats in each group. Model groups were fed edible raw coal mixed with peat and different formulas of zhijin roasted corn feed. Rats were sacrificed at 60, 120, and 180 days, and bilateral ovaries were harvested. Expression of apoptosis proteins Bcl?2 and Bax in ovarian granulosa cells was observed by immunohistochemistry. HE staining was used to observe ovarian tissue morphology and changes in follicle and corpus luteum numbers. Results There was no statistical significance in the protein expression of Bcl?2 in ovarian granulosa cells. The expression of Bax in each group was compared with that in the fluorosis group, and a significant difference was found at 120 and 180 days ( P <0.05). There were significant differences at 180 days between the two intervention groups ( P <0.05). Compared within each group, Bax expression in the fluorosis group gradually increased and the difference was significant ( P <0.05). There was a significant difference at 60 and 180 days for various elements ( P < 0.05). Over a prolonged time in the fluoride group, ovarian granulosa cells showed progressive edema, atretic follicles, and corpus luteum degradation, and mature follicles were decreased significantly. Ovarian granular cells in the two intervention groups showed rare mild edema and normal numbers of follicles at all levels. Atresia of follicles and degeneration of the corpus luteum were decreased significantly. Compared with the fluorosis group, there were significant differences in atresia follicles between control and multiple element + protein groups at 60 days, and the control group and two intervention groups at 120 and 180 days ( P <0.05). Conclusions Ca, Mg, Cu, Zn, and VD antagonize the reproductive toxicity of coal?burning fluorosis and reduce protein expression of Bax and the number of atresia follicles. And the effects of intervention combined with protein can be better.
王飞清,夏曙华,邹文兵,杨华,喻茂娟.营养元素和蛋白联合干预氟中毒对雌鼠卵巢功能作用[J].中国比较医学杂志,2018,28(7):52~57.
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