Objective To investigate the effect of astragaloside IV in combination with ferulic acid on the proliferation of cobalt chloride-induced hypoxic human umbilical vein endothelial cells (HUVECs) and its mechanism.Methods A hypoxic injury model of HUVECs was induced by cobalt chloride. CCK-8 and living cell staining techniques were used to detect cell proliferation, fluorescence staining was used to observe the morphological changes of cells, a scratch test was used to determine the migration ability of the cells, and a tube test was used to observe the effect of drugs on endothelial cells. Western blot was used to detect the expression of p-JAK2 and p-STAT3 proteins. Results Compared with the model group, the combination of astragaloside IV and ferulic acid significantly promoted the proliferation of HUVECs induced by cobalt chloride for 24 hours, improved morphological changes in cells induced by hypoxia, and promoted cell migration and angiogenesis. Concurrently, the protein expressions of p-STAT3 and p-JAK2 were upregulated.Conclusions Astragaloside IV combined with ferulic acid protects against cobalt chloride-induced hypoxia in HUVECs and promotes angiogenesis. The mechanism may be related to activating the JAK-STAT signaling pathway.