(1. 中国食品药品检定研究院,北京 102629;2. 浙江省医学科学院,杭州 310013;3. 广东省实验动物监测所,广州 510663)
R-33
(1. National Institutes for Food and Drug Control, Beijing 102629, China. 2. Zhejiang Academy of Medical Sciences, Hangzhou 310013. 3. Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663)
目的 验证团体标准中微卫星标记检测法应用于实验动物遗传质量检测,在3 家实验室是否具有较好的重复性。方法 3 家实验室同时应用12 个微卫星位点对6 份实验小鼠DNA 进行PCR 扩增,扩增产物经琼脂糖电泳和测序。结果 北京实验室获得清晰电泳条带,获得等位基因数72 个;浙江实验室获得清晰电泳条带,获得等位基因数72 个;广东实验室获得清晰电泳条带,获得等位基因数72 个。测序结果表明3 家实验室获得片段大小一致的等位基因。结论 微卫星标记检测法可以实现对实验动物的遗传质量评价,并在不同实验室获得较好的重复性。
Objective To verify the reproducibility of microsatellite detection for the genetic quality control of laboratory animals in three different laboratories. Methods DNA of six laboratory mice was amplified using 12 microsatellites in three laboratories. PCR products were analyzed by electrophoresis and sequencing. Results Clear electrophoresis bands and 72 alleles were obtained in the Beijing laboratory of National Institutes for Food and Drug Control (NIFDC), the Zhejiang laboratory, and the Guangdong laboratory. Sequencing results showed that the alleles identified were the same in the three different laboratories. Conclusions Microsatellites can be used for the genetic quality control of laboratory animals, with a good reproducibility between different laboratories.
王洪,戴方伟,王静,魏杰,杜江涛,黄树武,贾松华,岳秉飞.实验动物遗传质量控制微卫星标记检测法重复性研究[J].中国比较医学杂志,2019,29(1):84~89.
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