Objective To summarize the characteristics of two main individually ventilated cage (IVC) air supply modes in the construction of laboratory animal facilities, and analyze their different uses. Methods According to typical cases, the specific characteristics of the two air supply modes were analyzed in detail by five aspects: safety and reliability,space utilization, operation energy consumption, monitoring and regulation and initial investment. Results For each comparison item, the advantages and disadvantages of the two air supply modes were compared. Conclusions The two method of air supply had their individual advantages. Air supply mode A is suitable for small-scale facilities and projects that require a decentralized and flexible control of cage parameters. Air supply mode B is suitable for medium-sized and large-scale facilities.