Objective To explore a method to construct a rat model with qi stagnation and blood stasis chronic salpingitis, to study its feasibility and establish a method for its primary evaluation, and to lay the foundations for future experiments. Methods SD rats were subjected to predictable repeated stress stimulation (e.g., sound, light, electricity,and clip tail stimulation) and bacteria were injected into the oviduct to construct the model with qi stagnation and blood stasis chronic salpingitis. Symptoms, and observation, pathological, and blood rheology indexes were used to primarily evaluate the rat model. Results Compared with the control group, the combined qi stagnation and blood stasis with chronic salpingitis model group’s total mark rose significantly Qi( P <0. 01), 80% of the oviduct blood vessels exhibited swelling and distension, and 60% of their walls showed thickening. Pathological analysis revealed the infiltration of inflammatory cells in the oviduct lumens of the rat model with qi stagnation and blood stasis chronic salpingitis, and epithelial mucosal ablation was accompanied by substantial inflammatory cell infiltration surrounding the oviduct. Blood rheology analyses demonstrated very significantly increased plasma viscosity, whole blood viscosity, reduction viscosity, and erythrocyte rigidity index and aggregation index in the combined rat model group ( P < 0. 01). Conclusions Our comprehensive analysis reveals that the rat model with qi stagnation and blood stasis chronic salpingitis is roughly consistent with the dynamic characteristics of blood stasis and the pathological status of chronic inflammation, being similar to the human clinical manifestations. The method for constructing this model is feasible.