Brucella can cause acute and chronic infectious diseases in humans and animals, so it is one of themicroorganisms that must be excluded from experimental animals. With the passage of time, changes in conditions andadvances in technology, the original testing method and standards are no longer applicable for current Brucella detection,thus new method and standards for the detection of Brucella in laboratory animals need to be developed. This articleintroduces the background, legal basis, content compilation, and future prospects of the PCR test standards for Brucella inlaboratory animals, so that relevant personnel can better understand the standards and better carry out the detection ofBrucella in laboratory animals. This paper provides technical support for promoting the improved quality of laboratory animals and adapting to the needs of the international development of laboratory animals.