Heart failure is one of the leading causes of death for patients at the end stage of chronic cardiovasculardisease. The adverse remodeling of the myocardium plays a critical role in the occurrence of heart failure, and myocardialfibrosis is an important manifestation of adverse cardiac remodeling. Increasing evidence indicates that the epigeneticregulatory mechanisms play important roles in the occurrence and development of myocardial fibrosis. This review focuses onthe progress of research on epigenetic regulation, including DNA methylation, histone modification, and microRNA, in myocardial fibrosis.