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首页 > 过刊浏览>2019年第29卷第4期 >52-58. DOI:10.3969/j. issn. 1671 -7856. 2019. 04. 009
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不同剂量卵蛋白诱发BALB/ c 小鼠支气管哮喘模型的比较
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(1.北京中医药大学中药学院中药药理系,北京 102488;2. 中日友好医院中医肺病二部,国家中医药管理局重点研究室(肺病慢性咳喘),中医药防治过敏性疾病北京市重点实验室(BZ0321),中日友好医院呼吸中心;国家呼吸疾病临床研究中心,北京 100029)

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R-33


Comparison of asthma models induced by different doses of ovalbumin in BALB / c mice
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(1. School of Chinese Materia Medicine, Beijing University of Chinese Medicine, Beijing 102488, China.2. The Second Department of Traditional Chinese Medicine for Lung Disease, China-Japan Friendship Hospital, the Key Unit of SATCM Pneumonopathy Chronic Cough and Dyspnea, Beijing Key Laboratory of Prevention and Treatment of Allergic Diseases by Traditional Chinese Medicine (BZ0321), China-Japan Friendship Hospital Respiratory Center; National Respiratory Disease Clinical Research Center, Beijing 100029)

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    摘要:

    目的 探讨不同剂量卵蛋白(ovalbumin, OVA)对BALB/ c 小鼠支气管哮喘模型血清IgE?IL-4?IFN-γ 含量?支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)嗜酸性粒细胞(eosinophils,EOS)数量及肺组织病理学的影响,初步确定较为合适的OVA 造模剂量?方法 BALB/ c 小鼠随机分为正常组,模型1 组(OVA 20 μg),模型2 组(OVA 50 μg),模型3 组(OVA 100 μg),醋酸地塞米松组(OVA 100 μg)?除正常组外,其余各组小鼠分别腹腔注射相应剂量OVA 致敏,并以1% OVA 雾化激发,建立支气管哮喘模型?ELISA 法检测血清IgE?IL-4?IFN-γ 含量,瑞氏染色计算BALF 中EOS 数目,HE 染色观察肺组织病理学变化?结果 与正常组相比,各组小鼠血清IgE?IL-4?IFN-γ 及BALF 中EOS 均显著增加( P <0. 05),肺组织中均出现炎性细胞浸润?支气管上皮细胞变性?支气管平滑肌细胞增生等病理改变,在IgE?IL-4?IFN-γ 含量及肺组织病理变化方面以OVA 50 μg 组小鼠更为明显?结论 三种剂量OVA 均可成功诱发BALB/ c 小鼠出现哮喘,综合而言50 μg OVA 所诱发的哮喘模型更为明显,推荐作为BALB/ c 小鼠哮喘模型的适宜造模剂量?

    Abstract:

    Objective To investigate the effects of different doses of ovalbumin (OVA) on the serum levels ofIgE, IL-4, and IFN-γ, on the number of eosinophils (EOS) in bronchoalveolar lavage fluid (BALF), and on lunghistopathology in the BALB/ c mice with bronchial asthma, to determine the optimal OVA dose. Methods BALB/ c micewere randomly divided into a normal group, model 1 group (OVA 20 μg), model 2 group (OVA 50 μg), model 3 group(OVA 100 μg), and dexamethasone acetate group (OVA 100 μg). In addition to the normal group, the other groups ofmice were sensitized by intraperitoneal injection of the corresponding doses of OVA, and stimulated by 1% OVA atomizationto establish bronchial asthma models. The serum levels of IgE, IL-4, and IFN-γ were determined by ELISA. The number ofEOS in BALF was calculated by Wright staining, and the pathological changes of lung tissue were observed by HE staining.Results Compared with the normal group, the serum levels of IgE, IL-4, and IFN-γ and EOS in BALF were significantlyincreased in all groups ( P <0. 05). Inflammatory cell infiltration, bronchial epithelial cell degeneration, bronchial smoothmuscle cell hyperplasia, and other pathological changes were observed in the lung tissues. Especially, in the OVA 50 μggroup, the changes in the IgE, IL-4, and IFN-γ levels and lung histopathological changes were more pronounced. Conclusions All the three doses of OVA can induce asthma in BALB/ c mice successfully, and the asthma model inducedby 50 μg of OVA had more pronounced alterations. It is recommended that 50 μg of OVA be used as the appropriate dosage for establishing an asthma model in BALB/ c mice.

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高琴琴,丁子桐,李友林,史琦,苗丰,石璐,楚慧伦,孔德明,孙文燕.不同剂量卵蛋白诱发BALB/ c 小鼠支气管哮喘模型的比较[J].中国比较医学杂志,2019,29(4):52~58.

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  • 收稿日期:2018-09-14
  • 在线发布日期: 2019-05-10
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