(1. 湖北省肿瘤医院胃肠外科,武汉430079;2. 武汉大学中南医院胃肠外科,武汉430000)
R-33
(1. Department of Gastrointestinal Surgery, Hubei Cancer Hospital, Wuhan 430079, China.2. Department of Gastrointestinal Surgery, Central South Hospital, Wuhan University, Wuhan 430000)
目的 构建一种转移率高?操作简便?结果可靠的结肠癌肝转移模型,用于结肠癌转移防治的实验研究?方法 15 只Balb/ c 裸鼠平均分为3 组(A 组?B 组?C 组),5 只Balb/ c 小鼠单独为D 组,以细胞浓度2. 5×107/ mL 的HCT116?CT26 细胞悬液0. 2 mL 分别行脾种植保脾法及切脾法构建结肠癌肝转移模型,对比四组动物模型造模成功率及肝转移灶大小?数目及腹腔内转移情况?结果 A 组裸鼠造模成功率100%(5/5),肝及脾均成瘤,肝转移瘤数目较少,较分散,多分布于肝右叶,生存时间平均为(26. 6±3. 4) d;B 组裸鼠造模成功率40%(2/5),转移瘤分散于肝表面,体积较A 组大,生存时间平均为(36. 8±4. 2) d;C 组裸鼠造模成功率100%(5/5),肝及脾均成瘤,肝转移瘤数目较多,多个转移瘤融合成团,占据整个肝右叶,生存时间平均为(20. 2±2. 6) d;D 组肝未发现转移灶?三组裸鼠部分出现腹腔转移(A 组2 只,C 组3 只),均未出现心?肺?脑?肾转移灶?3 组裸鼠肝转移瘤组织细胞学形态符合腺癌的特征?结论 保脾法能获得较高的造模成功率,能有效模拟人类结肠癌细胞经血行转移至肝的途径和过程?
Objective To establish a mouse model of colon cancer liver metastasis with high metastasis rate,simple operation and reliable outcome, and to serve the studies on prevention and treatment of colon cancer metastasis.Methods Fifteen BALB/ c nude mice were divided into 3 groups of 5 mice each (A, B, and C), and group D consistingof 5 wild type BALB/ c mice. We established four colon cancer metastasis models: spleen planted, spleen preserving, andsplenectomy method using 0. 2 mL of HCT116 or CT26 cell suspension with a cell concentration of 2. 5 × 107/ mL,respectively. We compared the success rates of modeling in the four groups as well as intra-abdominal metastasis, and livermetastasis size and number. Results The success rate of the group A was 100% (5/5). The number of liver metastases inthe group A was low, dispersed, and located in the right lobe of the liver. The mean survival time was (26. 6 + 3. 4) days.The success rate of Group B was 40% (2/5). Metastatic tumors in the group B were scattered on the surface of the liver,and the volume was larger than the group A. The mean survival time was (36. 8 ±4. 2) days. The success rate of the groupC was 100% (5/5). The number of liver metastases in the group C was higher than in the other groups, and multiplemetastatic tumors fused into a mass occupying the right lobe of the liver. The mean survival time was (20. 2 ±2. 6) days.No metastasis was found in the group D. Peritoneal metastasis occurred in the three groups of nude mice (n =2 in Group A,n =3 in Group C) without heart, lung, brain, or kidney metastasis. The histopathological examination of liver metastasesconformed to the characteristics of adenocarcinoma. Conclusions The spleen preserving method shows the highest rate ofmodel establishment, and effectively simulates the route and process of human colon cancer cells travelling to the liver by the blood circulation.
李华驰,熊治国,谢敏,谈凯,殷涛,冯茂辉.结肠癌肝转移裸鼠模型的建立[J].中国比较医学杂志,2019,29(5):63~68.
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