红系祖细胞在造血缺陷斑马鱼体内的移植
作者:
  • 李志操

    李志操

    1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004
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  • 黄波 1

    黄波

    贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004
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  • 向文碧

    向文碧

    1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004
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  • 孙琮杰

    孙琮杰

    1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004
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  • 吴西军

    吴西军

    1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004
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  • 周艳华

    周艳华

    1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004
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  • 何志旭

    何志旭

    1. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004;2. 遵义医科大学附属医院儿科学教研室,贵州遵义 563003
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  • 舒莉萍

    舒莉萍

    1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004
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作者单位:

(1. 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室,组织工程与干细胞实验中心,临床医学院儿科学教研室,贵州省再生医学重点实验室,贵阳 550004;2. 中国医学科学院成体干细胞转化研究重点实验室,贵阳 550004;3. 遵义医科大学附属医院儿科学教研室,贵州遵义 563003)

中图分类号:

R-33


Transplantation of erythroid progenitor cells into hematopoietic deficient zebrafish
Author:
  • LI Zhicao

    LI Zhicao

    1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.
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  • HUANG Bo 1

    HUANG Bo

    National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.
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  • XIANG Wenbi

    XIANG Wenbi

    1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.
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  • SUN Congjie

    SUN Congjie

    1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.
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  • WU Xijun

    WU Xijun

    1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.
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  • ZHOU Yanhua

    ZHOU Yanhua

    1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.
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  • HE Zhixu

    HE Zhixu

    1. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.2. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003
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  • SHU Liping

    SHU Liping

    1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.
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Affiliation:

(1. National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique;Center for Tissue Engineering and Stem Cell Research; Department of Pediatrics, Clinical Medical College;Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China.2. Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004.3. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003)

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    摘要:

    目的 评估红系祖细胞在先天原始造血缺陷cloche-/ - 突变体斑马鱼体内的移植效果?方法 收集绿色荧光标记红系祖细胞的转基因系zTg(gata1:EGFP)斑马鱼胚胎,制备成单细胞悬液,经流式细胞仪分选出携带绿色荧光的gata1+细胞,利用显微注射技术将gata1+细胞移植到42hpf 的cloche-/ - 突变体斑马鱼心脏中,采用体视荧光显微镜追踪观察移植后的红系祖细胞在cloche-/ -突变体斑马鱼中的表达情况?结果 成功分选出携带绿色荧光的gata1+细胞,并在移植后2 h 可观察到携带绿色荧光的gata1+细胞逐渐增殖扩散且16 h 持续有绿色荧光表达?结论 红系祖细胞有重建造血的潜力,为进一步研究红系祖细胞移植后的功能鉴定提供实验依据?

    Abstract:

    Objective To evaluate the transplantation effect of erythroid progenitor cells in cloche-/ - mutantzebrafish with congenital primary hematopoietic deficiency. Methods The embryos of zebrafish zTg (gata1:EGFP) werecollected and prepared into a single cell suspension. The gata1+ cells carrying green fluorescence were separated by flowcytometry and transplanted into the hearts of 42 hpf cloche-/ - mutant zebrafish by microinjection. The expression of erythroidprogenitor cells in cloche-/ - mutant zebrafish was examined by stereo fluorescence microscopy. Results gata1+ cells withgreen fluorescence were successfully selected. The proliferation of gata1+ cells with green fluorescence was observed at 2 hafter transplantation, and 16 h of continuous green fluorescence expression was observed. Conclusions Erythroidprogenitor cells have a potential to reconstruct hematopoiesis and provide an experimental basis for the further study of the functional identification of erythroid progenitor cells after transplantation.

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李志操,黄波,向文碧,孙琮杰,吴西军,周艳华,何志旭,舒莉萍.红系祖细胞在造血缺陷斑马鱼体内的移植[J].中国比较医学杂志,2019,29(5):84~89.

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  • 收稿日期:2019-01-08
  • 在线发布日期: 2019-06-05
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