通过遗传多样性小鼠筛选EV71 致病相关基因

doi:10.3969/j. issn. 1671 -7856. 2019. 06. 002

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通过遗传多样性小鼠筛选EV71 致病相关基因
DOI:
                        10.3969/j. issn. 1671 -7856. 2019. 06. 002
                    
作者:
                        陈鑫陈鑫
北京协和医学院比较医学中心中国医学科学院医学实验动物研究所,国家卫健委人类疾病比较医学重点实验室,新发再发传染病动物模型研究北京市重点实验室,北京市人类重大疾病实验动物模型工程技术研究中心,国家中医药管理局人类疾病动物模型三级实验室,北京100021
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作者单位:(北京协和医学院比较医学中心中国医学科学院医学实验动物研究所,国家卫健委人类疾病比较医学重点实验室,新发再发传染病动物模型研究北京市重点实验室,北京市人类重大疾病实验动物模型工程技术研究中心,国家中医药管理局人类疾病动物模型三级实验室,北京100021)
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中图分类号:R-33
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Screening of EV71 pathogenicity-related genes using genetic diversity mice

Author:

CHEN Xin
CHEN Xin
Comparative Medicine Center,Peking Union Medical College(PUMC);Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS); Key Laboratory of Human Diseases Comparative Medicine,National Health Commission; Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases; Key Laboratory ofHuman Diseases Animal Model, State Administration of Traditional Chinese Medicine;Beijing 100021,China
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Affiliation:

(Comparative Medicine Center,Peking Union Medical College(PUMC);Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS); Key Laboratory of Human Diseases Comparative Medicine,National Health Commission; Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases; Key Laboratory ofHuman Diseases Animal Model, State Administration of Traditional Chinese Medicine;Beijing 100021,China)

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摘要:

目的通过遗传多样性小鼠资源(Genetic Diversity Mice Resources)筛选肠道病毒71 型(Enterovirus71,EV71)的致病相关基因,深入理解该病毒的致病机理,对临床诊断?治疗以及预后提供基础?方法 10⁷copiesEV71 病毒经腹腔感染4~6 周龄遗传多样性小鼠品系,收集血液和后肢骨骼肌样本,利用实时荧光定量PCR 技术分析病毒载量,之后利用The GeneMine 系统遗传学数据库分析感染数据,筛选获得EV71 致病相关基因?结果通过遗传多样性小鼠感染数据筛选到53 个与EV71 致病相关的基因?结论遗传多样性小鼠在模拟人群基因多样性?研究复杂性状疾病等方面具有较大的优势,是筛选致病相关基因理想的动物新资源?

关键词:肠道病毒71 型;致病相关基因;遗传多样性小鼠;手足口病

Abstract:

Objective The aim of this study was to screen enterovirus 71(EV71) pathogenicity-related genesusing genetic diversity mice to further understand the pathogenesis of the EV71, and lay the foundation for clinicaldiagnosis, treatment and prognosis. Methods Four-to-six week old genetic diversity mice were challenged with 10⁷copiesof enterovirus 71 by intraperitoneal injection. The blood and hind limb skeletal muscles were collected, and the viral loadwas determined by real-time quantitative PCR. Then, the GeneMine system genetic database was used to analyze theinfection data, and EV71 pathogenicity-related genes were screened. Results Fifty-three genes related to EV71 infectionwere screened using genetic diversity mouse infection data. Conclusions Genetic diversity mice have significantadvantages for simulating population genetic diversity and are a new resource to study complex trait diseases. Mostimportantly, they are an ideal and efficient resource for the screening of pathogenicity-related genes.

Key words:Enterovirus 71; pathogenicity-related host genes; genetic diversity mice; hand, foot and mouth disease

引用本文

陈鑫.通过遗传多样性小鼠筛选EV71 致病相关基因[J].中国比较医学杂志,2019,29(6):8~13.

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收稿日期:2018-12-30
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在线发布日期: 2019-07-16
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