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首页 > 过刊浏览>2019年第29卷第9期 >1-9. DOI:10.3969/j. issn. 1671 -7856. 2019. 09. 001
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甲醛对SH-SY5Y 细胞的损伤作用
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(国家卫生健康委员会人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京 100021)

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R-33


The damaging effect of formaldehyde on SH-SY5Y cells
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  • LI Hongwei

    LI Hongwei

    NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing 100021, China
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  • ZHANG Ling

    ZHANG Ling

    NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing 100021, China
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  • LI Zhuo

    LI Zhuo

    NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing 100021, China
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  • QIN Chuan

    QIN Chuan

    NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing 100021, China
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Affiliation:

(NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine; Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing 100021, China)

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    摘要:

    目的 利用SH-SY5Y 细胞系作为神经细胞的体外模型,探索甲醛对神经细胞的损伤作用,为甲醛损伤神经细胞的机制提供一定的科学依据?方法 将甲醛按不同作用浓度(对照组?0. 05 mmol/ L?0. 1 mmol/ L?0. 25 mmol/ L?0. 5 mmol/ L?1 mmol/ L)处理SH-SY5Y 人神经母细胞瘤细胞?采用细胞划痕实验?高倍镜检?CTG 细胞活性实验检测甲醛对细胞迁移能力?细胞形态及生存活性的影响;使用流式细胞术检测甲醛对细胞凋亡水平的影响;并通过透射电镜观察超微结构改变;使用免疫印迹法检测细胞内磷酸化tau 蛋白?Aβ 及线粒体裂解相关蛋白的表达水平?结果 0. 05 mmol/ L 甲醛即可抑制SH-SY5Y 细胞的迁移能力,0. 1 mmol/ L 作用4 h 即可降低细胞生存活性和增殖能力,上述改变与甲醛剂量和作用时间呈正相关;0. 05 mmol/ L 甲醛即可诱导SH-SY5Y 细胞的凋亡,可导致SH-SY5Y 超微结构线粒体的损伤;0. 25 mmol/ L 甲醛增加线粒体分裂,上调神经细胞磷酸化tau 蛋白的表达水平?结论 甲醛可通过抑制SH-SY5Y 细胞活性和增殖能力?降低其迁移能力?诱导凋亡等途径损伤神经细胞,可上调神经细胞tau 蛋白的磷酸化水平?

    Abstract:

    Objective To explore the toxic effects of formaldehyde using the human neuroblastoma SH-SY5Y cellline as an in vitro model of nerve cells, and to provide a scientific basis for the mechanism by which formaldehyde damagesneuronal cells. Methods SH-SY5Y neuroblastoma cells were treated with formaldehyde at different concentrations (controlgroup, 0. 05 mmol/ L, 0. 1 mmol/ L, 0. 25 mmol/ L, 0. 5 mmol/ L, and 1 mmol/ L). Cell scratch test, high-powermicroscopy, and CTG cell activity assay were used to detect the effect of formaldehyde on cell migration, morphology, andsurvival, respectively. Flow cytometry was used to detect the effect of formaldehyde on apoptosis, while ultrastructuralchanges were observed by transmission electron microscopy. Expression levels of phosphorylated tau, amyloid β, andmitochondrial fission-associated proteins were detected by western blotting. Results The migratory ability of SH-SY5Ycells was inhibited by even low (0. 05 mmol/ L) concentrations of formaldehyde, and the survival and proliferation of cellswas reduced by 0. 1 mmol/ L for 4 h. These changes were positively correlated with the dosage and duration of formaldehyde.Indeed, 0. 05 mmol/ L formaldehyde could induce the apoptosis and mitochondrial injury in the SH-SYSY cells, whereas,0. 25 mmol/ L formaldehyde increased mitochondrial fission and upregulated the expression level of phosphorylated tauprotein in the neuronal cells. Conclusions Formaldehyde can damage the neuronal cells, as it inhibites cell activity andproliferation of SH-SY5Y cells, reduces their migratory ability, and induces apoptosis. Moreover, formaldehyde upregulates the phosphorylation level of tau protein in neurons.

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李红威,张玲,李卓,秦川.甲醛对SH-SY5Y 细胞的损伤作用[J].中国比较医学杂志,2019,29(9):1~9.

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  • 收稿日期:2019-05-20
  • 在线发布日期: 2019-10-10
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