(山西医科大学实验动物中心,实验动物与人类疾病动物模型山西省重点实验室,太原 030001)
R-33
(Laboratory Animal Center, Shanxi Medical University; Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Models, Taiyuan 030001, China)
目的 筛选中国地鼠口腔鳞状细胞癌组织样本中具有差异表达的长链非编码RNA(long non-codingRNA,LncRNA),预测其靶基因,探究靶基因的生物学功能及富集的信号通路?方法 采用二甲基苯并蒽涂抹法建立中国地鼠口腔鳞状细胞癌模型,高通量测序技术构建LncRNA 差异表达谱,生物信息学技术筛选差异表达的LncRNA 并进行靶基因预测,GO 与KEGG 富集分析分别预测靶基因的生物学功能及富集的信号通路?结果 与正常组织样本相比,口腔鳞状细胞癌组织样本中筛选出54 个显著差异表达LncRNA(31 个上调,23 个下调)?功能性分析表明:与口腔癌相关的GO 条目73 条;KEGG 富集的信号通路25 条?结论 中国地鼠口腔鳞状细胞癌样本中差异表达的LncRNA 可能通过靶向调控其靶基因参与调控多种生物学功能及口腔癌相关的信号通路,在口腔鳞状细胞癌形成过程中可能发挥重要作用?
Objective To screen for differentially expressed long non-coding RNA (LncRNA) in oral squamouscell carcinoma tissue samples from Chinese hamsters and predict their target genes, and explore the biological functions and enrichment signaling pathways of these target genes. Methods The squamous cell carcinoma model was established inChinese hamsters by 9,10-dimethyl-1,2-benzanthracene smear method. The profile of differentially expressed LncRNA wasconstructed by high-throughput sequencing technology. Differentially expressed LncRNAs were screened by biosignaltechnology and target genes were predicted. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG)enrichment analyses were used to predict the biological function of differentially expressed genes and enriched signaling pathways, respectively. Results Compared with normal tissue samples, 54 differentially expressed LncRNAs werescreened from squamous cell carcinoma tissue samples, of which 31 were upregulated and 23 were downregulated.Functional analysis identified 73 GO entries associated with oral cancer, and 25 enriched KEGG signaling pathways.Conclusions Differentially expressed LncRNA in Chinese hamster oral squamous cell carcinoma samples likely play a rolein regulating various biological functions and oral cancer-related signaling pathways by targeting numerous genes, and may play an important role in the development of oral squamous cell carcinoma.
王晓堂,肖兰飞,高继萍,梁宇翔,轩瑞晶,闫晓如,高莉,宋国华.中国地鼠口腔鳞状细胞癌相关的长链非编码RNA 差异表达谱建立及应用[J].中国比较医学杂志,2019,29(11):7~15.
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