兔源多杀巴斯德杆菌基因分型研究
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广东省实验动物监测所,广东省实验动物重点实验室,广州 510663

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R-33


Genotyping of Pasteurella multocida isolated from laboratory rabbits
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Affiliation:

Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, China

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    摘要:

    目的 对实验动物设施普通级新西兰兔分离的多杀巴斯德杆菌分离菌株进行基因分型研究。 方法 2000-2018 年期间,基于分离培养和生化鉴定获得兔源多杀巴斯德杆菌 14 株,采用荚膜多重 PCR 方法和多位点序列分型方法对其进行荚膜血清型和基因型鉴定。 结果 荚膜多重 PCR 分型结果显示,兔源多杀巴斯德杆菌存在 荚膜血清型 A 型,占比 71. 4%(10 / 14),B 型占比 28. 6%(4 / 14);多位点序列分型分为 ST12、ST35、ST72、ST73、ST76 和 ST77 共 6 种 ST 型,且首次发现了新 ST76 型和 ST77 型。 结论 兔源多杀巴斯德杆菌基因分型存在复杂多样性, 荚膜血清型以 A 型为主,与呼吸道疾病相关,在生产繁育过程中应做好质量控制。

    Abstract:

    Objective To genotype clinical strains of Pasteurella multocida isolated from conventional rabbits bred in different laboratory animal facilities. Methods From 2000 to 2018, 14 strains of P. multocida were isolated from laboratory rabbits obtained from different laboratory animal facilities; these were then cultured and identified using biochemical testing. Using capsular multiplex PCR and multilocus sequence typing, serotypes and genotypes of the clinical strains of P. multocida were identified. Results Capsular multiplex PCR revealed capsular serotypes A and B among the 14 clinical strains of P. multocida, which comprised 71. 4% ( 10 / 14) and 28. 6% ( 4 / 14), respectively. Six sequence types (STs) were detected by multilocus sequence typing: ST12, ST35, ST72 and ST73, and two new types, ST76 and ST77, described for the first time here. Conclusions Genotype diversity was observed in P. multocida isolated from laboratory rabbits, and the capsular serotype A, which is associated with respiratory disease, accounted for a relatively high proportion of isolates. This study reinforces the importance of quality control for rabbit breeding.

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黄树武,王 静,潘金春,陈梅玲,张 钰,闵凡贵.兔源多杀巴斯德杆菌基因分型研究[J].中国比较医学杂志,2020,30(2):71~76.

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  • 收稿日期:2019-05-09
  • 在线发布日期: 2020-04-01
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