Mice are a small mammal model for scientific research. Before studying non-human primates and human embryos, it must be determined whether the experiment can be carried out in mice and then employ higher mammals. The limitation of the existing system is that mouse embryos can only be cultured in vitro to E6. 5. Establishment of a mouse embryo in vitro culture system is very important to understand the critical events and molecular mechanisms of embryo peri- implantation. The development of single-cell RNA sequencing technology provides new possibilities for in vitro time-lapse culture of mouse embryos. Here, we introduce an in vitro time-lapse culture system and 3D culture system for mouse embryo implantation, the application of single-cell RNA sequencing technology in the field of embryos, and its possible future direction of development.