Objective To study the inhibitory effects of Fuzheng Kang-Ai (FZKA) decoction on the migration and invasion of human ovarian carcinoma (HO-8910PM) cells and the undeR_Lying mechanisms. Methods Forty-eight SD rats were randomly divided into a control group, low-dose FZKA group (4. 725 g / (kg·d)), medium-dose FZKA group (9. 45 g / (kg·d)), and high-dose FZKA group (18. 9 g / (kg·d)). The drug was administered by gavage once per day for 7 days. The decoction was isolated and used to incubate HO-8910PM cells, and changes in the related indices were detected by the cell scratch test, transwell assay, ELISA, real-time quantitative PCR, and Western blot. Results Compared with the control group, after 24 hours of low -, medium-, and high-dose FZKA decoction treatment, HO-8910PM cell mobility and invasion were significantly lower ( P< 0. 01). Compared with the control group, after 12 -, 24 -, and 48 h of low -, medium-, and high-dose FZKA decoction treatment of HO-8910PM cells, FN protein expression decreased significantly (P<0. 05 or P< 0. 01). Compared with the control group, after 24 h of low -, medium- and high-dose FZKA decoction treatment of HO-8910PM cells, E-cadherin mRNA expression increased (P<0. 01), while vimentin, N-cadherin mRNA, TGF-β1, and p-SmaD₃ expression decreased significantly (P<0. 01). Conclusions FZKA can inhibit the migration and invasion of HO-8910PM cells, by inhibiting TGF-β1 / SmaD₃ pathway activation and further regulation of the EMT process.