天台乌药对 TNBS 诱导的溃疡性结肠炎模型大鼠的抗炎作用研究
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作者单位:

1.浙江工业大学 长三角绿色制药协同创新中心,杭州 310014; 2.杭州医学院 安全性评价研究中心, 杭州 310053; 3.浙江中医药大学 药学院,杭州 310053

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R-33


The effect of Tiantai Radix Linderae on a rat model of ulcerative colitis induced by TNBS
Author:
Affiliation:

1.Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China. 2. Center of Safety Evaluation, Hangzhou Medical College, Hangzhou 310053. 3. College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053

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    摘要:

    目的 探究天台乌药对 TNBS 诱导的溃疡性结肠炎模型大鼠的抗炎作用。 方法 36 只雄性 SD 大鼠随机分为正常对照组、模型对照组、阳性对照组、乌药醇提物低、中、高剂量组。 采用 2,4,6 -三硝基苯磺酸 (TNBS)50%乙醇溶液灌肠建立 UC 大鼠模型。 造模同时开始灌胃给予天台乌药醇提物干预 9 d,实验期间通过观测大鼠体重、便血等疾病症状表现及结肠组织病变程度等指标评价乌药给药后的药效作用;通过检测大鼠结肠组织髓过氧化物酶(MPO)活性、血清中 IL-6、TNF-α 含量及外周血中 Treg 等淋巴细胞亚群比例等指标初步探讨其可能机制。 结果 与模型对照组比较,天台乌药各剂量组大鼠疾病症状及结肠组织病变均有所减轻;外周血中 Treg 占辅助性 T 细胞比例均有所升高;结直肠重量及单位长度重量显著降低(P<0. 05)。 天台乌药中剂量组结直肠长度显著增加(P<0. 05)。 天台乌药各剂量组大鼠血清中 IL-6 含量均显著降低(P<0. 01);天台乌药低、中剂量组大鼠血清中 TNF-α 含量及结肠组织中 MPO 活性均显著降低(P<0. 05)。 结论 天台乌药能够改善 TNBS 诱导的 UC 模型大鼠的疾病症状及组织病理学改变,具有良好的抗 UC 作用。 乌药的抗 UC 作用可能与其降低 IL-6 等炎性细胞因子含量有关,具体机制仍有待进一步研究。

    Abstract:

    Objective To investigate the anti-inflammatory effect of LREE on a rat model of ulcerative colitis (UC) induced by TNBS. Methods Thirty-six male Sprague-Dawley rats were randomly divided into 6 groups: the normal control group (NC), model control group ( TNBS), positive control group ( SASP), and WY-L, WY-M, and WY-H groups. The rat model of UC was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS), and the Linderae Radix ethanol extracts (LREE) were given by intragastric administration for 9 days. During the experimental period, disease symptoms, including body weight and stool features, and the colon histomorphology of UC model rats were observed. The activity of myeloperoxidase (MPO) in the colon was measured, the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were determined, and the proportion of regulatory T cells (Treg) and other lymphocyte subsets in the peripheral blood was detected by flow cytometry. Results The result showed that all three doses of LREE could significantly improve the symptoms of hematochezia and weight loss in UC model rats and reduce the serum levels of IL-6 ( P< 0. 05). Furthermore, LREE reduced the weight and length of the colon and rectum and improved the macroscopic changes, such as intestinal mucosal congestion and intestinal wall shortening and thickening, and histomorphological changes, including intestinal epithelial cell injury and immune cell infiltration. Additionally, low and middle doses reduced the serum level of TNF-α and activity of MPO in the colon (P<0. 05) and increased the proportion of Treg cells in the peripheral blood of model rats. Conclusions These findings suggested that LREE exerted anti-UC effects on the rat model induced by TNBS and that the mechanism might be associated with the inhibition of inflammatory cytokines, such as IL-6 and TNF-α.

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赖慧敏,黄敏聪,楼招欢,谢 锋,潘 伟,徐 聪,杨正标,秦 荔,张立将.天台乌药对 TNBS 诱导的溃疡性结肠炎模型大鼠的抗炎作用研究[J].中国比较医学杂志,2021,31(2):37~44.

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  • 收稿日期:2020-04-28
  • 在线发布日期: 2021-04-07
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