Objective To summarize popular stimulation factors, model duration, and main behavioral tests used in the chronic unpredicted mild stress (CUMS) model of depression, so as to provide a reference for the use of CUMS in depression research. Methods Literature related to CUMS models from the past 10 years was searched for in PubMed databases and screened according to certain criteria. The animals, stressors, and duration of CUMS models were summarized. Results Male rats are most often used in CUMS models. Seven to nine stressors should be chosen for an experiment and should not be repeated within 3-7 days. Among the stimulation factors, deprivation of food or water, damp bedding, cold water swimming, tilt cages, and changes in light rhythm are recommended. Three to four weeks are recommended for the model duration. Behavioral tests included sucrose preference test, open field test and force swimming test. Conclusions This study provides a useful reference for the use of CUMS in models of depression regarding the animals, stimulation factors, and model duration.