1.山西医科大学实验动物中心,实验动物与人类疾病动物模型山西省重点实验室,太原 030001; 2.山西医科大学附属精神卫生医院,太原 030001
R-33
1.Laboratory Animal Center, Shanxi Medical University; Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Models, Taiyuan 030001, China. 2. Mental Health School Affiliated to Shanxi Medical University, Taiyuan 030001
目的 研究槲皮素对 Tca-8113 细胞增殖、迁移和侵袭功能的影响及 PI3K/ AKT 通路的调控作用。 方法 体外培养 Tca-8113 细胞,将细胞分为对照组和槲皮素(50 μmol / L)处理组。 采用 CCK-8 实验检测细胞增殖能力;Transwell(添加基质胶)检测细胞迁移(侵袭)情况;实时荧光定量 PCR( qRT-PCR)检测细胞中 PTEN、AKT、 FOXO1 以及 BCL2L11 mRNA 表达,蛋白质免疫印迹法(Western blot)检测 FOXO1、p-FOXO1、AKT、p-AKT、PTEN 和 BCL2L11 蛋白表达。 结果 与对照组相比,槲皮素处理组 Tca-8113 细胞的增殖、迁移及侵袭能力显著降低,PTEN 和 BCL2L11 mRNA 和蛋白表达水平均显著上调(P<0. 05),FOXO1 和 AKT 磷酸化水平显著下调(P<0. 01),但 AKT 和 FOXO1 mRNA 表达水平无显著差异。 结论 槲皮素可抑制 Tca-8113 细胞增殖、迁移及侵袭,其机制可能是槲皮素促进 PTEN 的表达,负调控 PI3K/ AKT 通路减弱 FOXO1 的磷酸化水平,引起 BCL2L11 的表达增加实现的。
Objective To investigate the effect of quercetin on cell proliferation, migration, and invasion and PI3K/ AKT pathway regulation in the oral cancer cell line Tca-8113. Methods Tca-8113 cells were cultured in vitro and divided into a control group and quercetin ( 50 μmol / L) treatment group. A CCK-8 assay was used to detect cell proliferation; a Transwell assay ( with Matrigel) was used to detect cell migration ( invasion); quantitative real-time polymerase chain reaction was used to detect the expression of PTEN, AKT, FOXO1, and BCL2L11 mRNA; and Western blot was used to detect FOXO1, p-FOXO1, AKT, p-AKT, PTEN, and BCL2L11 protein expression. Results Compared with the control group, the proliferation, migration, and invasion abilities of Tca-8113 cells in the quercetin treatment group were significantly reduced; the PTEN and BCL2L11 mRNA and protein expression levels were significantly up-regulated (P< 0. 05); and the phosphorylated FOXO1 and AKT levels were significantly down-regulated (P< 0. 01). However, there was no significant difference in the AKT and FOXO1 mRNA expression levels between the two groups. Conclusions Quercetin can inhibit the proliferation, migration, and invasion of Tca-8113 cells. In terms of the underlying mechanism, quercetin may promote the expression of PTEN and negatively regulate the PI3K/ AKT pathway to reduce the phosphorylation level of FOXO1, causing an increase in the expression of BCL2L11.
肖兰飞,高继萍,闫晓如,王晓堂,续国强,宋国华.槲皮素对口腔癌 Tca-8113 细胞增殖、迁移和侵袭的影响及 PI3K/ AKT 信号通路的调控作用[J].中国比较医学杂志,2021,31(6):30~34.
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