Objective An animal model of 70% partial hepatectomy + liver dual arterial blood supply (LDABS) was established. Methods Seventy-six rats were divided into a training group (n= 40) and a model group (n= 36). In the model group, 70% partial hepatectomy (left lobe and middle lobe of liver) + double artery blood supply surgery of the liver was performed under a microscope. The general status of rats was observed after surgery, and the success and 1 week survival rates were calculated. Results Through simulated surgery in the training group, the learning curve was crossed. The surgery duration of the model group was (65. 3 ± 6. 56) min, and the vascular anastomosis time was (11. 1 ± 2. 53) min. During the surgery, there were no obvious side injuries and less bleeding, of no more than 0. 8 mL, was observed. One week later, the modeling success rate was 88. 9% ( 32 / 36), and the rats were in good condition. Conclusions Surgery consisting of 70% hepatectomy + LDABS in rats under a microscope is relatively complicated. However, after a period of microsurgery training, the improvements and innovation of the surgical mode, especially vascular anastomosis and perioperative nursing, resultsed in a high success rate and strong repeatability of the model, laying the foundation for further research on its application in liver regeneration, acute liver failure, and liver transplantation.