Objective The ginsenosides, polysaccharides and adenosine contents in the Dendrobium-ginseng- paecilomyces batmoth-ginger Capsule were determined. To study the immune function effect of Dendrobium-ginseng- paecilomyces batmoth-ginger Capsule in CI/ F1 (BALB/ c×ICR) female mice. Methods Ginsenoside and polysaccharide were determined by colorimetric method and adenosine was determined by High Performance Liquid Chromatography (HPLC). Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule in distilled water was administered by oral gavage to female mice at a dose of 260, 530 or 1580 mg / kg. After 30 d, the bodyweight, immune organ index, spleen lymphocyte proliferation ability, delayed type hypersensitivity, serum hemolysin, natural killer (NK) cell activity, antibody generation cellular level and the changes of macrophage phagocytic function in the abdominal cavity were evaluated for 10 female animals in each group at the end of the study. Results The ginsenoside, polysaccharide and adenosine contents in Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule were 1. 495 g / 100 g, 12. 90 g / 100 g and 73. 8 mg / 100 g, respectively. In the 1580 mg / kg group, the cellular immune function ( spleen lymphocyte proliferation and delayed type hypersensitivity reaction) in the mice were enhanced, the level of antibody generation was elevated and the macrophage cellular and phagocytic activities were increased. Conclusions Under the conditions of this experiment, the Dendrobium- ginseng-paecilomyces batmoth-ginger Capsule enhanced the immune function in mice.