Objective To establish a method of ovarian heterotopically transplantation in KM，ICＲ，BALB/c and C57BL/6J mice． Methods the ovaries were used as donor from 10d，20d and 4w of KM，ICＲ，BALB/C and C57BL/6J mice，and were grafted to the 4w accepters of same strains． All accepters were bred about 4-week-old，then mated with the same strain male mice to identify the reproductive capacity of the donors． Ｒesults the grafted ovaries could be restored reproduction，and gotten the normal pups when mated with same strain males． Conclusion It is feasible that ovarian heterotopically transplantation in the same mouse strains of different age phases．