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首页 > 过刊浏览>2022年第32卷第2期 >1-9. DOI:10. 3969 / j.issn.1671-7856. 2022. 02. 001
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尿酸性肾病大鼠模型建立的研究
作者:
  • 严美霞

    严美霞

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120
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  • 霍 帅

    霍 帅

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;4.河南省人民医院肾内科,华中阜外医院肾内科,郑州 450003
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  • 田瑞敏

    田瑞敏

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120;5.广东省中医药防治难治性慢病重点实验室,广州 510006;6.广东省中医证候临床研究重点实验室,广州 510006
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  • 徐 鹏

    徐 鹏

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120;5.广东省中医药防治难治性慢病重点实验室,广州 510006;6.广东省中医证候临床研究重点实验室,广州 510006
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  • 黎 创

    黎 创

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120;5.广东省中医药防治难治性慢病重点实验室,广州 510006;6.广东省中医证候临床研究重点实验室,广州 510006
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  • 胡天祥

    胡天祥

    2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120
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  • 陈达豪

    陈达豪

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120
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  • 陈小玲

    陈小玲

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120
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  • 毛 炜

    毛 炜

    1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120;6.广东省中医证候临床研究重点实验室,广州 510006
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作者单位:

1.省部共建中医湿证国家重点实验室(广州中医药大学第二附属医院),广州 510120;2.广州中医药大学第二临床医学院, 广州 510120;3.广东省中医院肾内科,广州 510120;4.河南省人民医院肾内科,华中阜外医院肾内科,郑州 450003; 5.广东省中医药防治难治性慢病重点实验室,广州 510006;6.广东省中医证候临床研究重点实验室,广州 510006

中图分类号:

R-33


Establishment of a rat model of uric acid nephropathy
Author:
  • YAN Meixia

    YAN Meixia

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.
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  • HUO Shuai

    HUO Shuai

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003.4. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central Fuwai Hospital, Zhengzhou 450003.
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  • TIAN Ruimin

    TIAN Ruimin

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.5. Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangzhou 51006.
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  • XU Peng

    XU Peng

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.5. Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangzhou 51006.
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  • LI Chuang

    LI Chuang

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.5. Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangzhou 51006.
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  • HU Tianxiang

    HU Tianxiang

    2.Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.
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  • CHEN Dahao

    CHEN Dahao

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.
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  • CHEN Xiaoling

    CHEN Xiaoling

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.
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  • MAO Wei

    MAO Wei

    1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006.6. Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou 51006
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Affiliation:

1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, (the Second Clinical Medical College of Guangzhou University of Chinese Medicine), Guangzhou 510006, China. 2. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central China Fuwai Hospital, Zhengzhou 450003. 3. Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006. 4. Department of Nephrology, Henan Provincial People’s Hospital, Department of Nephrology of Central Fuwai Hospital, Zhengzhou 450003. 5. Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangzhou 51006. 6. Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou 51006

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    摘要:

    目的 建立合理、稳定的尿酸性肾病大鼠模型,为筛选及研究治疗尿酸性肾病提供病理模型。 方法 实验大鼠随机分为 3 个造模组和正常组。造模组分别予以 750 mg / kg 氧嗪酸钾联合 150 mg / kg 低剂量尿酸(M-A 组)、750 mg / kg 氧嗪酸钾联合 300 mg / kg 中剂量尿酸(M-B 组)、750 mg / kg 氧嗪酸钾联合 600 mg / kg 高剂量尿酸 (M-C 组),正常组(Control 组)予以 0. 3%羧甲基纤维素钠(100 g / mL),连续灌胃 4 周后检测血尿酸、血肌酐、尿素氮、甘油三酯、胆固醇等指标的变化以及肾的病理学改变。 结果 与正常组比较,3 个模型组的血尿酸明显高于正常组(P<0. 01,P<0. 05,P<0. 05);M-B 组、M-C 组的血肌酐显著升高(P<0. 05,P<0. 01);M-B 组的甘油三酯明显低于正常组(P<0. 05);肾病理评分 M-B 组、M-C 组的肾病理评分明显高于正常组(P<0. 01);Masson 染色形态学显示,3 个模型组与正常组比肾损伤明显(P<0. 05,P<0. 01,P<0. 01)。免疫组化炎症 CD68结果显示,与正常组比,3 个模型组表达量增加(P<0. 05,P<0. 01,P<0. 01);E-Cadherin 表达与正常组相比,M-B、M-C 组表达量明显降低 (P<0. 01);α-SMA 表达与正常组相比,3个模型组表达量增加(P< 0. 05,P< 0. 01,P< 0. 01)。 结论 氧嗪酸钾 (750 mg / kg)联合中剂量尿酸(300 mg / kg)可作为构建尿酸性肾病大鼠模型较为理想的方法。

    Abstract:

    Objective Establish a reasonable and stable rat model of uric acid nephropathy to provide a pathological model for screening, research, and treatment of uric acid nephropathy. Methods Experimental rats were randomly divided into three model groups and a control group. Model mice were administrated orally with 750 mg / kg oteracil potassium combined with 150 mg / kg low dose uric acid (M-A), 750 mg / kg oteracil potassium combined with 300 mg / kg medium dose uric acid (M-B), or 750 mg / kg oteracil potassium combined with 600 mg / kg high dose uric acid (M- B). After continuous intragastric administration for 4 weeks, changes in blood uric acid, blood creatinine, urea nitrogen, triglycerides, cholesterol and other indicators as well as pathological changes of the kidneys were observed for 4 weeks. Results Compared with the control group, the serum uric acid of the three model groups was significantly higher (P< 0.01, P<0.05, P<0.05), serum creatinine of the M-B and M-C groups was increased significantly (P<0.05, P<0. 01), triglycerides of the M-B group were significantly lower (P<0.05). Renal pathology scores of M-B and M-C groups were significantly higher those of the control group (P<0.01). Masson staining of morphology showed that, in comparison with the control group, kidney damage was more obvious in the three model groups ( P< 0.05, P< 0.01, P< 0.01 ). Immunohistochemical staining of inflammation marker CD68 showed that, compared with the control group, its expression was increased of in three model groups (P<0. 05, P<0. 01, P<0. 01). Expression of epithelial cell marker E-Cadherin was significantly reduced in M-B and M-C groups in comparison with to the control group ( P< 0.01 ). Expression of myofibroblast marker protein α-SMA was increased the three model groups compared with the control group (P<0.05, P< 0.01, P<0.01). Conclusions Oteracil potassium (750 mg / kg) combined with the middle dose of uric acid (300 mg / kg) is ideal to establish a rat model of uric acid nephropathy.

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严美霞,霍 帅,田瑞敏,徐 鹏,黎 创,胡天祥,陈达豪,陈小玲,毛 炜.尿酸性肾病大鼠模型建立的研究[J].中国比较医学杂志,2022,32(2):1~9.

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  • 收稿日期:2021-01-28
  • 在线发布日期: 2022-04-12
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