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首页 > 过刊浏览>2022年第32卷第4期 >1-6. DOI:10. 3969 / j.issn.1671-7856. 2022. 04. 001
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四物合剂对缩宫素所致原发性痛经模型小鼠的影响研究
作者:
作者单位:

1.中国中医科学院西苑医院基础医学研究所,北京 100091;2.国家中医心血管病临床医学中心,北京 100091; 3.中药药理北京市重点实验室,北京 100091;4.解放军总医院第六医学中心,北京 100048; 5.中国中医科学院西苑医院病理科,北京 100091

中图分类号:

R-33


Effect of Siwu mixture on primary dysmenorrhea model mice induced by oxytocin
Author:
  • LIU Zixin

    LIU Zixin

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • JIA Feifan 4

    JIA Feifan

    the Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048
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  • MENG Hongxu

    MENG Hongxu

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • HAN Xiao

    HAN Xiao

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • LI Lingmei

    LI Lingmei

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • YOU Yue

    YOU Yue

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • WANG Aoao

    WANG Aoao

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • WANG Ziyan

    WANG Ziyan

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • YANG Bin 5

    YANG Bin

    Department of Pathology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091
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  • LI Lei

    LI Lei

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • FU Jianhua

    FU Jianhua

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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  • LIU Jianxun

    LIU Jianxun

    1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091.
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Affiliation:

1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. National Clinical Research Center for Chinese Medicine Cardiology, Beijing 100091. 3. Beijing Key Laboratory of Chinese Materia Pharmacology, Beijing 100091. 4. the Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048. 5. Department of Pathology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091

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    摘要:

    目的 研究四物合剂对原发性痛经小鼠模型的影响,探讨其可能的作用机制。 方法 健康 SPF 级 KM 雌性小鼠 60 只,随机分为 4 组,分别为正常组、模型组、痛经宝颗粒组、四物合剂组。 采用雌激素联合缩宫素诱导的原发性痛经小鼠模型,适应性喂养 3 d 后,小鼠皮下注射苯甲酸雌二醇 2 mg / kg,连续给药 10 d。 第 4 天开始, 正常组和模型组给予等剂量蒸馏水,阳性药痛经宝颗粒组按 4 g / kg 给药,四物合剂组按 8 mL/ kg 给药,连续给药 7 d。 以注射缩宫素后 30 min 内扭体次数和扭体潜伏期为主要观测指标,考察四物合剂的镇痛作用,测定血清中的 超氧化物歧化酶(SOD)、丙二醛(MDA);酶联免疫吸附测定法(ELISA)检测子宫组织前列腺素F2α(PGF2α )、白介素 6(IL-6)、肿瘤坏死因子 α(TNF-α)含量;制备子宫病理切片观察腺体增生、炎性细胞浸润情况,免疫组化检测环氧化酶 2(COX-2)表达。 结果 与正常组相比,模型组 30 min 内扭体次数明显增加、扭体潜伏期显著缩短(P<0. 01), 血清 MDA 水平显著增加(P<0. 01),子宫组织 PGF2α 、TNF-α 水平明显增加(P<0. 01),IL-6 含量增加(P<0. 05);子宫内膜腺体扩张呈乳头状增生,间质水肿,炎性细胞增生、浸润,COX-2 表达增强。 与模型组相比,阳性药组、四物合剂组 30 min 内扭体次数降低(P<0. 05,P<0. 01),四物合剂组扭体潜伏期增加(P<0. 05),阳性药组、四物合剂组 血清 MDA 水平降低(P<0. 01),子宫组织 PGF2α水平降低(P<0. 01,P<0. 05),IL-6、TNF-α 含量降低(P<0. 05),腺体增生减少,间质紧密,炎性细胞浸润减少,COX-2 表达降低。 结论 四物合剂能显著减少原发性痛经小鼠 30 min 扭体次数,延长扭体潜伏期,其作用机制可能与降低血清 MDA 含量、子宫组织中 PGF2α 、IL-6、TNF-α 水平以及 COX-2 表达,改善组织病理学变化有关,值得进一步深入研究。

    Abstract:

    Objective Siwu mixture is derived from Siwu decoction, a classical Chinese medicine prescription, which is widely used in a variety of gynecological diseases. However, There are few studies on the therapeutic effect of Siwu mixture on primary dysmenorrhea. This study aims to further explain its therapeutic effect. Methods Sixty healthy animals were divided into four groups: the control group, model group, Siwu mixture group and Tongjingbao granule group. The primary dysmenorrhea mice model was induced by estrogen combined with oxytocin. After 3 days of adaptive feeding, mice were subcutaneously injected with estradiol benzoate 2 mg / kg for 10 days. The normal and the model group received the same dosage of distilled water from the 4th day. The Tongjingbao granule group was given 4 g / kg for 7 days. The Siwu mixture group was given 8 mL/ kg for 7 days. The analgesic effect of Siwu mixture was studied by writhing and the latency of writhing within 30 min after oxytocin injection. The serum contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were determined. ProstaglandinF2α (PGF2α ), interleukin-6 ( IL-6) and tumor necrosis factor-α (TNF-α) were measured in tissue homogenates by ELISA method. Uterine pathological sections were prepared to observe glandular hyperplasia and inflammatory cell infiltration, and the expression of cyclooxygenase-2 ( COX-2 ) was detected by immunohistochemistry. Results Compared with result in the control group, the body twisting in the model group increased (P<0. 01) and serum MDA increased (P<0. 01). Uterine tissue PGF2α , TNF-α and IL-6 increased. In the model group, endometrial glands were dilated and papillary hyperplasia, interstitial edema, inflammatory cell proliferation and infiltration were observed; COX-2 expression was enhanced. The number of writhing events within 30 min in Tongjingbao granule group and Siwu mixture group decreased compared with controls (P<0. 05, P<0.01); the writhing latency in the Siwu mixture group increased (P<0.05) and the level of serum MDA in the positive drug group and Siwu mixture group decreased (P< 0.01). Conclusions Siwu mixture could significantly reduced the 30 min writhing times and prolonged the writhing latency of primary dysmenorrhea mice. The mechanism may be related to reducing the content of serum MDA and PGF2α , TNF-α and IL-6 in uterine tissue and COX-2 expression are related to the improvement of histopathological changes.

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刘子馨,贾飞凡,孟红旭,韩 笑,李玲美,尤 越,王奥奥,王紫艳,杨 斌,李 磊,付建华,刘建勋.四物合剂对缩宫素所致原发性痛经模型小鼠的影响研究[J].中国比较医学杂志,2022,32(4):1~6.

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  • 收稿日期:2021-12-13
  • 在线发布日期: 2022-06-20
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