广西中医药大学第三附属医院神经内科,广西 柳州 545000
R-33
Department of Neurology, the Third Affiliated Hospital of Guangxi University of Chinese Medicine, Liuzhou 545000, China
目的 研究缺氧诱导因子 1α(HIF-1α)对小胶质细胞 M1 极化的影响及其影响机制。 方法 将小胶质细胞(BV-2 细胞)随机分为 6 个组:Control 组和 10、50、100、200、500 μg / L 重组 HIF-1α 蛋白刺激处理组。 采用荧光共聚焦法观察各组 BV-2 细胞的形态变化;采用免疫蛋白印迹法定量分析重组 HIF-1α 蛋白刺激处理后 NF-κBp65、p-STAT1 和 TRAF6 蛋白含量变化。 结果 与对照组相比,重组 HIF-1α 蛋白刺激后小胶质细胞胞体变大,呈圆形或吞噬状,突起变粗变短;胞内 NF-κB p65、TRAF6 蛋白较对照组显著增加,不同浓度重组 HIF-1α 蛋白刺激处理组对比结果有显著差异。 结论 HIF-1α 可刺激小胶质细胞 M1 极化,不同浓度重组 HIF-1α 蛋白刺激处理有量效关系,其机制可能与通过 TLR4 / Myd88 / NF-κB 通路调节 TRAF6 和 NF-κB 活化有关。
Objective To study the effect of hypoxia-inducible factor 1α (HIF-1α) on microglia M1 polarization and its mechanism. Methods Microglia (BV-2 cells) were randomly divided into six groups: control, 10, 50, 100, 200 and 500 μg / L recombinant HIF-1α groups. Morphological changes of BV-2 cells were observed by fluorescence confocal microscopy. Changes of nuclear factor (NF)-κB p65, p-STAT1 and TRAF6 protein contents after recombinant HIF-1α protein stimulation were quantitatively analyzed by Western blot. Results Compared with the control group, microglia cells stimulated by recombinant HIF-1α protein became larger, round or phagocytic, and their processes became thicker and shorter. Intracellular NF-κB p65 and TRAF6 proteins were significantly increased compared with the control group, and the result of groups with varying concentrations of recombinant HIF-1α protein stimulation were significantly different. Conclusions HIF-1α can stimulate microglial M1 polarization and there was a dose-effect relationship between different concentrations of recombinant HIF-1α, which may be related to the regulation of TRAF6 and NF-κB activation through the TLR4 / Myd88 / NF-κB pathway.
张雪儿,安红伟.缺氧诱导因子1α对小胶质细胞M1极化的影响及其机制研究[J].中国比较医学杂志,2022,32(9):34~38.
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