Objective The purpose of this study is to comprehensively elucidate the anti-hepatic fibrosis mechanism of ( Glycyrrhiza Uralensis and Salvia Miltrorrhiza,GUSM) from the perspective of endogenous metabolites. Methods Hepatic fibrosis rat model was established by subcutaneously injecting CCl₄ solution. The efficacy of GUSM was evaluated by liver morphological indexes and HE method . We analyzed, screened and identified the metabolic profiles of rats from Normal group, Model group, GUSM group and Positive group by PCA and OPLS-DA method using UPLC-TOFMS. Results A total of 16 potential biomarkers in both positive and negative mode associated with hepatic fibrosis were screened and identified. The metabonomics analysis revealed that Taurocholic acidf Glycocholic acid, Arachidonic acid,and so on were greatly disturbed. Conclusions GUSM prescription showed anti fibrosis effect on rats through regulating metabolic pathways, mainly about Arachidonic acid metabolism. This study is useful to better understand and analyze the anti hepatic fibrosis mechanism of GUSM prescription on HF rats using metabonomics.