With the in-depth study of animal welfare,as an important part of 3R optimization principle,research on humane intervention points in animal experiments has gradually attracted international attention. Choosing the right humane intervention points and interventions is the key to safeguard animal welfare. Humane intervention points refers to the time when animals should be intervened during animal experiments. Humane endpoint is a type of humane intervention points, and euthanasia is an implementation method of humane intervention points. At present, a number of standards and regulations on animal welfare have been issued in our country, but few on humane intervention points. This paper will review the guides and literature reports on humane intervention points of animal experiment, and provide helpful references on animal welfare for animal experiment researchers.