Objective To conduct standardized identification of a strain of Rodentibacter pneumotropicus isolated from the respiratory tract of mice in Jiangxi Province according to the identification and collection procedures for laboratory animals and zoonosis preservation at the Center of Bacteria and Virus Species Preservation, Chinese Academy of Medical Sciences, National Pathogenic Microorganism Resource Bank, and the requirements of national standard strain identification. Methods According to the requirements of GB14922. 1 and GB14926. 12, routine health-monitoring animals were examined and pathogen strains were isolated. The samples were transported to the National Bacterial and Viral Species Preservation Center at low temperatures for further classification and identification of pathogenic microorganisms by means of colony, cell characteristics, physiological and biochemical characteristics, phylogenetic analysis of 16S Rrna steward gene, and genome sequence analysis. Whole-genome sequencing result were compared and analyzed, and the pathogenicity was evaluated by artificial infection of mice. Results According to GB14926. 2, one strain of R. pneumophilus was isolated from a batch of mice from Ganzhou, Jiangxi Province, and the animals had no other obvious abnormalities. The isolated strain was similar to NCTC 8141 (R. pneumotropicus) colony, cell, and physiological and biochemical characteristics. The strainhad 98. 37% homology with the NCTC 8141 16S rRNA gene sequenceand formed a branch with R. pneumotropicus NCTC 8141 in the phylogenetic tree, with 96% self-promotion data value. On genome sequence analysis, the ANI and dDDH values between the isolated and R. pneumotropicus type strain NCTC 8141 were 97. 97% and 80. 3%, respectively, higher than the international standard classification limits of 95% and 70%. The clinical symptoms and pathological changes to the lung tissue were similar to those of the type strain NCTC 8141 infected with ICR (Institute of Cancer Research) mice via nasal drops. Conclusions The isolate was named R. pneumotropicus Jiangxi strain by the National Laboratory Animal and Zoonosis Preservation sub-center, and the strain was stored in a standardized way and numbered CCPM-B-B-006-2209-2.