2 型糖尿病db/ db 小鼠骨强度和肠道内容物菌群的变化
作者:
作者单位:

1.昆明医科大学实验动物学部,昆明 650500;2.昆明医科大学科技成果孵化中心,昆明 650504

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R-33


Changes of bone strength and gut microbiota in type 2 diabetic db / db mice
Author:
Affiliation:

1. Department of Laboratory Animal Science, Kunming Medical University, Kunming 650500, China. 2. Incubation Center of Scientific and Technological Achievements, Kunming Medical University, Kunming 650504

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    摘要:

    目的 观察db/ db 小鼠血糖、骨强度和肠道菌群的变化。 方法 20 周龄雄性db/ db 小鼠及野生型小鼠各6 只,检测空腹血糖,行三点弯曲实验检测股骨力学指标,采用16S rRNA 高通量测序技术对小鼠肠道内容物基因V3-V4 区进行测序及生物信息分析。 结果 db/ db 组小鼠体重(P<0. 01)和血糖(P<0. 05)均显著升高。db/ db 小鼠胫骨刚度、最大位移、最大能量吸收、最大载荷均显著低于WT 小鼠(P<0. 01)。db/ db 小鼠α 多样性指标中的Chao1 指数、ACE 指数和observed species 均显著降低(P<0. 05);两者肠道细菌群落组成存在显著差异(ANOSIM:P=0. 02)。db/ db 小鼠毛螺菌属(Lachnospira)显著降低(P<0. 01);变形菌门(Proteobacteria)、δ-变形菌纲(Deltaproteobacteria)、脱硫弧菌目(Desulfovibrionales)显著升高(P<0. 05),Alloprevotella 属极显著升高(P<0. 01)。 结论 本研究结果表明db/ db 小鼠高血糖且骨强度降低,是T2DOP 的适宜动物模型。db/ db 小鼠与WT 小鼠肠道菌群的多样性及特定菌群丰度存在差异,为研究肠道菌群与糖尿病性骨质疏松提供了理论基础。

    Abstract:

    Objective To observe changes in blood glucose, bone strength, and intestinal microflora in db/ db mice. Methods Six male db/ db mice and six wild-type(WT)mice at 20 weeks of age were used to assess fasting blood glucose. A three-point bending test was performed to assess mechanical parameters of the femur. 16S rRNA high-throughput sequencing technology was used to sequence the V3-V4 gene region of intestinal contents and analyze the bioinformation. Results The body weight(P< 0. 01) and blood glucose(P< 0. 05) of mice in the db/ db group were increased significantly. Tibial stiffness, maximum displacement, maximum energy absorption, and maximum load of db/ db mice were significantly lower than those of WT mice(P<0. 01). The Chao1 index, ACE index, and observed species in the α-diversity index of db/ db mice were decreased significantly(P<0. 05). There were significant differences in the intestinal bacterial community composition between the two groups(ANOSIM: P=0. 02). Lachnospira was significantly decreased in db/ db mice(P<0. 01). Desulfovibrionales, Proteobacteria, and Deltaproteobacteria were significantly increased(P<0. 05), and Alloprevotella was increased significantly(P<0. 01). Conclusions db/ db mice with hyperglycemia and decreased bone strength are suitable animal models of T2DOP. The diversity of intestinal flora and the abundance of specific flora were different between db/ db and WT mice, which provides a theoretical basis to study intestinal flora and diabetic osteoporosis.

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赵玉兰,杨 艺,李进涛,王佩锦,角建林,郑 红.2 型糖尿病db/ db 小鼠骨强度和肠道内容物菌群的变化[J].中国比较医学杂志,2023,33(6):1~7.

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  • 收稿日期:2022-02-24
  • 在线发布日期: 2023-08-18
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