Laboratory animal barrier contamination by pathogenic microorganisms during the operation of environmental facilities seriously affects research and animal health or leads to biosafety incidents, interference of experimental result, or interruption of experiments. However, elimination of contamination during their long-term operation is difficult ,and can occur due to careless management or operational details. Contamination by pathogenic microorganisms is a major problem for laboratory animal managers. After engaged in laboratory animal barrier facility management for many years, The author deeply felt the importance of details management to prevent infection by pathogenic microorganism. Therefore, summarized of the details problem combined facility layout with staff management based on years of working experience, which in order to be discussed and used for reference.