IBS-D 大鼠肠道菌群-短链脂肪酸代谢轴变化特点及丁酸钠干预作用研究
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1.广州中医药大学第二附属医院,广州 510120;2.广州中医药大学东莞医院,广东 东莞 523000;3.省部共建中医湿证国家重点实验室,广州 510120

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R-33

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Alterations in gut microbiota-short-chain fatty acid axis in rats with diarrheapredominant irritable bowel syndrome and the effect of sodium butyrate
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1. the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. 2. Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000. 3. State Key Laboratory of Dampness Syndrome of Chinese Medicine, Guangzhou 510120

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    摘要:

    目的 探索腹泻型肠易激综合征 (diarrhea-predominant irritable bowel syndrome,IBS-D) 大鼠模型的肠道菌群-短链脂肪酸代谢轴变化特点,以及探讨丁酸钠对该轴的影响。 方法 将17 只Wistar 大鼠随机分为3组,采用4%醋酸灌肠联合束缚应激诱导IBS-D 大鼠模型,造模期间予以丁酸钠腹腔注射,采用16S rRNA 技术检测大鼠粪便菌群,液相色谱-质谱联用检测大鼠粪便短链脂肪酸含量。 结果 与正常组比较,模型组体重下降,腹壁撤退反射(abdominal withdrawal reflex,AWR)评分、粪便含水率明显升高,肠道益生菌相对丰度下降,布劳特氏菌属(Blautia)相对丰度明显升高,粪便乙酸含量显著增加,丁酸、戊酸含量明显下降;与模型组比较,丁酸钠组大鼠体重增加,AWR 评分、粪便含水率明显降低,布劳特氏菌属(Blautia)相对丰度显著下降,而粪便丁酸、戊酸含量显著升高。 结论 丁酸钠可通过改善肠道菌群-短链脂肪酸代谢轴失调缓解IBS-D 大鼠腹泻及结肠内脏高敏。

    Abstract:

    Objective To explore the alterations in the gut microbiota-short-chain fatty acid metabolism axis in a rat model of diarrhea-predominant irritable bowel syndrome (IBS-D), and to explore the effect of sodium butyrate on this axis. Methods Seventeen Wistar rats were divided randomly into three groups. IBS-D was induced by 4% acetic acid enema combined with restraint stress. Sodium butyrate was injected intraperitoneally during the modeling period. The fecal flora was detected by 16S rRNA technology, and the fecal short-chain fatty acid content was detected by liquid chromatography mass spectrometry. Results Rat body weight, the relative abundance of intestinal probiotics, and fecal butyric acid and valeric acid contents were decreased in rats in the model group compared with the normal group, while the abdominal withdrawal reflex score, fecal water content, and relative abundance of Blautia spp. were significantly increased. Treatment with sodium butyrate significantly increased rat body weights and fecal butyric acid and valeric acid contents, and decreased the AWR score, fecal water content, and relative abundance of Blautia spp. , compared with the model group. Conclusions Sodium butyrate can alleviate diarrhea and colon visceral hypersensitivity in IBS-D rats by improving the imbalance in the gut microbiota-short-chain fatty acid metabolic axis.

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占 凯,吴皓萌,郑 欢,秦书敏,杨元明,黄绍刚. IBS-D 大鼠肠道菌群-短链脂肪酸代谢轴变化特点及丁酸钠干预作用研究[J].中国比较医学杂志,2023,33(9):16~24.

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  • 收稿日期:2022-12-16
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  • 在线发布日期: 2023-11-09
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