Abstract: Objective To investigate the effects of low doses of bisphenol A (BPA) and di (2-ethyl) hexyl phthalate (DEHP) on aldo-keto reductase 1C3 (AKR1C3) expression in adult SD rats. Methods Fifty-six adult male SD rats were randomly divided into seven groups (n =8 rats in each group) and administrated BPA (10, 30 and 90 μg/ kg, i. g. ), DEHP (30, 90 and 270 μg/ kg, i. g. ), or the vehicle once a day for 4 weeks. The animals were sacrificed on the day after the last treatment. Blood was collected, and prostate tissues were dissected and categorized into different lobes. Levels of AKR1C3 in serum and prostate were measured by an enzyme-linked immunosorbent assay, and AKR1C3 expression in each prostate lobe was analyzed by immunohistochemistry. Results After BPA administration, AKR1C3 expression in the ventral prostate was increased, and a significant difference was found in the 90 μg/ kg group (P<0. 05). AKR1C3 protein expression in the dorsal prostate was increased, and a significant difference was found in the 10 μg/ kg group (P<0. 01, P< 0. 001). After DEHP administration, the serum level of AKR1C3 in the 270 μg/ kg group was significantly higher than that in the control group (P<0. 001), the AKR1C3 level in the ventral prostate was significantly higher than that in the control group (P<0. 05, P<0. 01), AKR1C3 protein expression was increased, and a significant difference was found in the 270 μg/ kg group (P<0. 05). The AKR1C3 level in the dorsal prostate of the 30 and 90 μg/ kg groups was higher than that in the control group (P<0. 001, P<0. 05). Conclusions Low-dose BPA and DEHP promotes AKR1C3 expression in the prostate of adult SD rats, but the sensitivities of ventral and dorsal prostate lobes to BPA and DEHP are different.