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首页 > 过刊浏览>2023年第33卷第10期 >45-53. DOI:10. 3969 / j.issn.1671-7856. 2023. 10. 007
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黄芩素介导自噬经由miR-449a/ HDAC1 轴抑制喉癌细胞增殖及迁移的机制研究
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作者单位:

1.西南医科大学附属中医院,耳鼻咽喉头颈外科,四川 泸州 646000;2.西南医科大学中西医结合学院,四川 泸州 646000

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R-33


Effects of baicalin on proliferation and apoptosis of laryngeal cancer cells via the miR-449a/ HDAC1 axis
Author:
Affiliation:

1.Department of Otolaryngology, Head and Neck Surgery, Affiliated Hospital of Traditional Chinese Medicine, Southwest Medical University, Luzhou 646000, China. 2. College of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou 646000

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    摘要:

    目的 探究黄芩素抑制喉癌细胞增殖及迁移的机制。 方法 采用Hep-2 细胞为研究对象。MTT 法检测各组细胞增殖活力;划痕实验检测细胞迁移;流式细胞术检测细胞凋亡情况;Western blot 检测各组细胞中Beclin-1、LC3II/ I、p62 及HDAC1 的表达水平;RT-PCR 检测细胞miR-449a 的表达水平;生物信息学软件分析miR-449a 与HDAC1 的靶向结合位点,双荧光素酶报告基因实验鉴定;透射电子显微镜观察自噬小体数量。 结果 黄芩素显著抑制了喉癌细胞的增殖和迁移,并诱导细胞凋亡;黄芩素增加了细胞内Beclin-1 及LC3II 的蛋白表达水平,减少了p62 及LC3I 的蛋白表达水平;黄芩素显著增加喉癌细胞miR-449a 的表达水平,降低HDAC1 的蛋白表达量;HDAC1 与miR-449a 存在结合作用;黄芩素联合miR-449a mimic 处理喉癌细胞,可以更显著的抑制细胞增殖及HDAC1 的蛋白表达水平,以及更为有效地上调miR-449a 的相对表达量和诱导自噬。 结论 黄芩素可以介导自噬经由miR-449a/ HDAC1 轴抑制喉癌细胞增殖及迁移。

    Abstract:

    Objective To explore the mechanism of baicalein inhibiting the proliferation and migration of laryngeal cancer cells. Methods Proliferation of Hep-2 laryngeal cancer cells was measured by MTT assays. Cell migration was assessed by a scratch assay. Apoptosis was detected by flow cytometry. Western blot was used to measure protein expression levels of Beclin-1, LC3II/ I, p62 and HDAC1. The expression level of miR-449a was measured by RTPCR. The binding sites of miR-449a and HDAC1 were analyzed by bioinformatics and identified by dual luciferase reporter assays. The number of autophagosomes was observed by projection microscopy. Results Baicalein significantly inhibited the proliferation and migration of laryngeal cancer cells, and significantly induced apoptosis. Baicalein increased the protein expression levels of intracellular autophagy-related genes Beclin-1 and LC3II, but decreased the protein expression levels of p62 and LC3I. Baicalein significantly increased the expression level of miR-449a in laryngeal cancer cells and decreased protein expression of HDAC1. Binding was observed between HDAC1 and miR-449a. Cotreatment of laryngeal cancer cells with Baicalein and an miR-449a mimic more significantly inhibited cell proliferation and HDAC1 protein expression, and more effectively upregulated miR-449a expression and induced autophagy. Conclusions Baicalein mediated autophagy and inhibited the proliferation and migration of laryngeal cancer cells via the miR-449a/ HDAC1 axis.

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王剑,孙永东,周兴玮,刘雷,童兴科,陈龙,何娴.黄芩素介导自噬经由miR-449a/ HDAC1 轴抑制喉癌细胞增殖及迁移的机制研究[J].中国比较医学杂志,2023,33(10):45~53.

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  • 收稿日期:2022-11-03
  • 在线发布日期: 2023-12-29
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